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An electrochemical biosensor, its preparation method, application and detection method

A biosensor, electrochemical technology, applied in the field of biosensors, to achieve the effects of low energy consumption, good selectivity and low cost

Active Publication Date: 2016-09-14
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Electron mediators are in the related research. However, there are still few reports on the strategy of using mimetic enzymes to bind electronic mediators to amplify signals.

Method used

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  • An electrochemical biosensor, its preparation method, application and detection method
  • An electrochemical biosensor, its preparation method, application and detection method
  • An electrochemical biosensor, its preparation method, application and detection method

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Embodiment Construction

[0042] The present invention will be described in detail below according to the accompanying drawings, which is a preferred embodiment among various implementations of the present invention.

[0043] The invention relates to a method for synthesizing gold nano particles. The base complementation between DNA molecules is used to assemble an electrochemical biosensor on the surface of a gold electrode, and an electronic medium is used to drive the catalysis experiment of simulating DAN enzyme to detect potassium ions. Gold nanoparticles (AuNPs) were synthesized using the sodium citrate reduction method, and a ferrocene (Fc)-labeled probe was assembled with a probe capable of forming a mimetic DNase by utilizing the complementary base pairing between DNA sequences. On the surface of the gold electrode, an electrochemical biosensor based on ferrocene-driven simulated DNA enzyme catalysis and gold nanoparticle amplification was prepared. This sensor realized the detection of the sen...

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Abstract

The invention relates to an electrochemical biosensor as well as a preparation method, application and a detection method. According to the preparation method, gold nanoparticles (AuNPs) are synthesized by virtue of a sodium citrate reduction method, a probe marked with ferrocene (Fc) and a probe capable of forming simulated DNA (deoxyribonucleic acid) enzyme are assembled to the surface of a gold electrode by virtue of a complementary pairing action of base among DNA sequences, so as to prepare the electrochemical biosensor capable of catalyzing the simulated enzyme and amplifying the gold nanoparticles based on the driving of the ferrocene. By utilizing the electrochemical biosensor, the sensitivity, the specificity and the stability of a target potassium ion are detected.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to constructing an electrochemical biosensor based on ferrocene-driven simulated DNA enzyme catalysis and gold nanoparticle amplification to realize sensitive detection of potassium ions. Background technique [0002] In recent years, DNase with catalytic properties has become a research hotspot because of its easy preparation, assembly, hydrolysis and thermal stability. In the field of DNase research, it was further found that the G-quadruplex horseradish peroxide mimic DNase has peroxidase-like properties, and it can catalyze the reaction with hydrogen peroxide as the substrate. Based on the catalytic properties of simulated enzymes, simulated DNases have been widely used to amplify detection signals. In addition, traditional enzymes (such as horseradish peroxidase, glucose oxidase, etc.) have been proved to have a good electron transfer effect with electron mediators. Electr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 王广凤陈玲
Owner ANHUI NORMAL UNIV
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