Specific primer based kit for identifying five common cryptoleste stored grain insects

Abstract

The invention discloses a specific primer based kit for identifying five common cryptoleste stored grain insects. The kit provided by the invention comprises at least one pair of the following 5 pairs of primers: a primer pair 1 (sequence 1 and sequence 2), a primer pair 2 (sequence 3 and sequence 4), a primer pair 3 (sequence 5 and sequence 6), a primer pair 4 (sequence 7 and sequence 8) and a primer pair 5 (sequence 9 and sequence 10). Experiments show that compared with a traditional morphological identification method, the cryptoleste stored grain insect species identification method using the kit provided by the invention does not need morphological taxonomy base and realizes the identification of non-adult state; andcompared with other molecular biological identification methods, the method simplifies the molecular detection steps for identification of common cryptoleste stored grain insects, has the characteristics of simple operation, short time consumption, high sensitivity, stability and visual results. The invention lays a foundation for further research on identification of sibling species of cryptoleste.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a kit for identifying or assisting in identifying five kinds of common stored-grain beetles. Background technique [0002] The thorax belongs to the order Coleoptera, the family Laemophloeidae, and the genus Cryptolestes. There are more than 20 species of this genus recorded at home and abroad, of which about 10 species are harmful to stored grain. , and the five species that are widely distributed in the world are the rusty red beetle, the long horned beetle, the Turkish beetle and the Cape beetle. [0003] Coleoptera insects have the largest number and variety of stored grain pests, and the most prominent quarantine significance. The flat rice beetle is a group of economically important group of Coleoptera stored grain pests. Its larvae and adults can harm grains, beans, and oil plants. A variety of agricultural products and their processed products, which can cause serious economic...

AI Technical Summary

Problems solved by technology

The result of restriction fragment length polymorphism PCR technology is relatively accurate, but the workload of screening the required restriction endonucleases is relatively large

Up to now, there are no reports on the molecular identification techniques for the five common grain beetles in the world, namely, the rusty beetle, the long-horned beetle, the microbeetle, the Turkish beetle and the cape beetle

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