A kind of lignin peroxidase preparation removes the method for drug and personal care category pollutant

A peroxidase and personal care technology, applied in the field of biological treatment of pollutants, can solve the problems of low concentration of PPCPs, and achieve the effect of efficient degradation and high efficiency

Active Publication Date: 2016-03-23
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the concentration of PPCPs in urban sewage is very low at present, and the literature r

Method used

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  • A kind of lignin peroxidase preparation removes the method for drug and personal care category pollutant
  • A kind of lignin peroxidase preparation removes the method for drug and personal care category pollutant
  • A kind of lignin peroxidase preparation removes the method for drug and personal care category pollutant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Table 1 shows the water quality parameters of the actual sewage collected in this example. The concentration of the four PPCPs selected in the reaction system is 0.01mM, the activity of the added LiP is 0.0065U / mL, and the concentration of H 2 o 2 The concentration is 0.01mM, the VA concentration is 1.0mM, and the pH of the reaction system is adjusted to 3.0, 3.7, 4.6, 5.0, 5.3, 6.2, 7.0 with phosphoric acid-citrate buffer solution, and the temperature is controlled at 25°C and shaken at constant temperature After reacting for 90 minutes at a speed of 150 rpm in the incubator, the residual amount of pollutants was detected. The result obtained is as figure 1 Shown: the reaction has a higher degradation rate between pH 3 and 5.5, and the degradation efficiency of the four PPCPs is the largest at 99% (degradation rate > 96%) when the pH is 4.6.

[0042] Table 1 The property parameters of the filtered sewage treatment plant effluent

[0043] compound

[0044]...

Embodiment 2

[0046] The four PPCPs selected in the reaction system represent pollutant concentrations of 0.01mM, the LiP activity added to the reaction system is 0.0065U / mL, the pH of the reaction system is adjusted to 4.6 with phosphoric acid-citrate buffer, and veratrol ( VA) concentration was 1.0mM, and different amounts of hydrogen peroxide were added to adjust the concentration of hydrogen peroxide in the reaction system to be 0.005mM, 0.01mM, 0.02mM, 0.05mM, 0.1mM, 0.2mM, 0.5mM, respectively, and The temperature was controlled at 25° C., and the residual amount of pollutants was detected after reacting for 90 minutes at a speed of 150 rpm in a constant temperature shaking incubator. The result obtained is as figure 2 Shown: H 2 o 2 When the concentration of LiP is 0.01mM, the catalytic degradation rate of LiP to 4 kinds of pollutants is greater than 90%, if H 2 o 2 When the concentration increases by 10 times (0.10mM), the removal rate of pollution is obviously reduced (less tha...

Embodiment 3

[0048] The four PPCPs selected in the reaction system represent pollutant concentrations of 0.01mM, the LiP activity added to the reaction system is 0.0065U / mL, and the pH of the reaction system is adjusted to 4.6 with phosphoric acid-citrate buffer, H 2 o 2 The concentration of VA was 0.01mM, and different amounts of veratrol were added to the sewage to adjust the VA concentration of the reaction system to 0.5mM, 1mM, 2mM, 3mM, 4mM, 5mM, and the temperature was controlled at 25°C. After reacting for 90 minutes at a rotating speed of 150 rpm in a shaking incubator, the residual amount of pollutants was detected. Investigate the results of different VA concentrations such as image 3 As shown: the VA concentration of about 1mM can maintain high enzyme activity and stability. At this time, the degradation rate of LiP enzyme to the four PPCPs reaches 90%. If the concentration of VA continues to increase, the VA and pollutants will Competing for enzyme binding sites, resulting i...

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Abstract

The invention discloses a method for removing pharmaceutical and personal care product (PPCP) pollutants by using a lignin peroxidase preparation, belonging to the technical field of biological treatment of pollutants. The method comprises the following steps: (1) acquiring sewage, determining the pH value, adding a phosphoric acid-citrate buffer solution while keeping the pH value of the water sample at 3-5.5, analyzing the PPCPs content in the sewage, and adding PPCPs into the water sample until the pollutant concentration in the sewage is 0.01mM; (2) regulating the temperature of the reaction system to 25-40 DEG C, sequentially adding 0.5-1.5mM veratryl alcohol and 0.0055U/mL above LiP enzyme into the reactor, and adding 0.01-0.1mM H2O2 into the reaction system to start the reaction; and (3) keeping the reactor in the oscillation or stirring state. The separated and purified peroxidase preparation is added into the wastewater containing PPCP pollutants to efficiently remove the PPCP pollutants.

Description

technical field [0001] The invention belongs to the technical field of pollutant biological treatment, and more specifically relates to a method for efficiently removing pollutants of drugs and personal care products in water by a lignin peroxidase preparation. Background technique [0002] Pharmaceuticals and personal care products (PPCPs) is a general term for a large class of compounds, representative drugs such as antibiotics, antipyretic, analgesic and anti-inflammatory drugs, contraceptives, antihypertensive drugs, tranquilizers, etc., and daily care products such as personal skin care And cosmetics, fragrances, preservatives, detergents, sunscreens, hair styling agents, dental care products, etc. PPCPs are widely used and discharged as people's daily necessities. These compounds enter the environment through direct or indirect discharge, domestic sewage discharge, sludge reuse, landfill, livestock and poultry and aquaculture. In 1999, Daughton and Term, with the supp...

Claims

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Application Information

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IPC IPC(8): C02F3/00
Inventor 毛亮高士祥罗思强
Owner NANJING UNIV
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