Oral care compositions
An oral care and composition technology, applied in the field of oral care compositions, can solve the problems of poisoning bacterial cells and being unable to be used in the same way
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[0078] a. Mixed Species Biofilm Test :mix species Bacteria were grown as follows: Actinomyces viscosus (ATCC# 31346). for mixing species Determination, the source of the bacteria was inoculated with Actinomyces viscosus, Lactobacillus casei (Lactobacillus casei) (ATCC#334), Streptococcus oralis (ATCC #35037), Fusobacterium nucleatum (ATCC#10953 ) and Veilonella parvula (ATCC #17745) continuous culture chemostat. This mixed culture was maintained in a dedicated complex medium at 37°C in a continuous culture chemostat.
[0079] Mixing performed on saliva-coated HAP discs (scHAP) species biofilm. After saliva coating, discs were inoculated with 1 ml of the mix from the above continuous culture chemostat species The culture, which was diluted with 0.5x TSB to an OD610 of ~0.2. Plates were incubated at 37°C for 48 hours to allow biofilm formation.
[0080] Discs containing pre-formed biofilms were transferred to new 24-well culture plates and 1 ml of sterile 0.5x TSB ...
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