Rapid propagation method of Cuba orchids

An orchid, fast technology, applied in the field of rapid propagation of Cuban orchids through leaf induction, can solve problems such as difficulty and low success rate

Inactive Publication Date: 2014-08-27
太原大学外语师范学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of asexual reproduction of Cuban orchids is to use roots to induce callus formation, and then undergo differentiation and rooting culture. The difficulty of this method is that it is difficult to induce callus formation with roots and the success rate is low.
And the method for inducing rapid propagation of Cuban orchids by leaves has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Select the buds on the bulbs of healthy Cuban orchid plants as explants, scrub them with detergent and rinse them with tap water for 30 minutes, and then wash them with 0.1% HgCl under sterile conditions. 2 Surface disinfection for 3 minutes, then surface disinfection with 75% alcohol for 30 seconds, and finally rinsed with sterile water, placed in MS+5.0mg / L 6-BA+1.5mg / L NAA+30g / L sucrose and 7g / L Cultivate seedlings in L agar and a medium with a pH of 5.8. The culture conditions are: 17-20°C, light intensity 1300-1500Lux, and photoperiod 15h / d. When the seedlings grow to 2-3 leaves, the leaves are 7 -8cm, with scissors the blades are cut into 0.8-1.0cm long blades. Then the leaves were sterilized and transferred to MS+6-BA 1.0mg / L+2,4-D 0.5mg / L+7g / L agar+30g / L sucrose, and the pH was 5.8 in the induction medium for callus Induction culture, culture temperature 17-19 ℃, dark culture, culture for 65 days, callus induction rate was 80.7%. Place the induced callus in MS...

Embodiment 2

[0017] Take the buds on the bulbs of Cuban orchid plants as explants, scrub them with detergent and rinse them with tap water for 30 minutes, and wash them with 0.1% HgCl under aseptic conditions. 2 Surface disinfection for 3 minutes, then surface disinfection with 75% alcohol for 30 seconds, and finally rinsed with sterile water, placed in MS+5.0mg / L 6-BA+1.5mg / L NAA+30g / L sucrose and 7g / L L agar, PH 5.8 culture medium, culture conditions: 20-23 ℃, light intensity 1000-1250Lux, photoperiod 15h / d, when the seedlings grow to 2-3 leaves, the leaves are 7 When -8cm, blade is cut into the long blade of 1.1-1.2cm with scissors. Then the leaves are sterilized and transferred to MS+6-BA 0.5mg / L+2,4-D 0.3mg / L+7g / L agar+30g / L sucrose, and the pH is 5.8 in the induction medium for callus Induction culture, culture temperature 20-23 ℃, dark culture, culture for 60 days, callus induction rate was 80.7%. Place the induced callus in MS+6-BA 1.0mg / L+2,4-D 0.4mg / L+sucrose 30g / L+agar 7g / L, p...

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PUM

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Abstract

The invention discloses a rapid propagation method of Cuba orchids, which comprises the following steps: preparing asexual reproduction leaves; carrying out induced culture to obtain calluses; carrying out multiplication culture to obtain a large amount of calluses; carrying out differentiation culture and rooting culture; hardening seedlings; and transplanting test-tube seedlings. According to the method, the leaves of the test-tube seedling are taken as explants; calluses are formed by inducing rapid propagation of the leaves; and finally the Cuba orchid seedlings are obtained by carrying out multiplication culture, differentiation culture and rooting culture of the calluses. By using the method, the induction rate of the calluses is greater than 80 percent; the differentiation rate of the seedlings is greater than 60 percent; and the rooting rate of the seedlings is greater than 85 percent; the method solves the practical problems of low induction rate of the calluses, low differentiation rate and low rooting rate of the seedlings in the existing asexual reproduction technology of the Cuba orchids, and has realistic significance on large-scale seedling culture of the Cuba orchids.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of Cuban orchids, in particular to a method for inducing rapid propagation of Cuban orchids through leaves. Background technique [0002] Orchids are known as the king of flowers because of their beautiful and unique shape, huge flowers, bright colors, rich colors, and long flowering period. Cuban orchids are the best of orchids. Cuban orchids are epiphytic flowers with uniaxial stems, and the plants rarely germinate lateral buds. Conventional propagation methods are difficult to meet market demand, and tissue culture is the most effective way to rapidly propagate Cuban orchids. In 1960, Morel proposed a method for in vitro clonal propagation of orchids. With the popularization of orchids, the research on the rapid propagation system of orchids has gradually deepened. Various asexual reproduction techniques have become a research hotspot. However, due to biological differenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 方岩王培军夏罗宏薛斌
Owner 太原大学外语师范学院
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