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Method for obtaining haploid regenerated plant by eggplant anther culturing

A technology for regenerating plants and eggplant flowers, applied in the agricultural field, can solve the problems of low eggplant anther culture efficiency, achieve the effects of reducing browning, solving low induction rate, and enriching germplasm resources

Inactive Publication Date: 2014-09-17
NANJING INST OF VEGETABLE SCI
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for cultivating eggplant anthers to obtain haploid

Method used

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Examples

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Embodiment Construction

[0039] In this example, the hybrid first-generation eggplant combinations S8847 and S8889 with comprehensive and excellent traits were used as test materials, and haploid plants were obtained by the method of the present invention. The implementation process is as follows:

[0040] 1. Materials

[0041] In the first ten days of May, the flower buds of eggplant and Simendou were collected at 10:00 am on a sunny day during the full flowering period of the test materials. The stages of pollen development were examined under microscope by acetic acid magenta staining, and the correlation between the stages of pollen development and anther morphology was determined. Select flower buds whose pollen development is in the middle and late stages of mononuclei. The length of the bud is 12.0-13.5mm, the diameter of the bud is 6.5-7.5mm, the calyx wraps the corolla, and the apex is slightly opened to show the top of the corolla. The anthers are greenish-yellow; 24-26d, that is, flower b...

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Abstract

The invention relates to a method for obtaining a haploid plant by eggplant anther culturing, which belongs to the technical field of agriculture. The method comprises the following steps: by identification of a pollen development stage, selecting a flower bud; sterilizing the flower bud; grafting anther; pretreating; culturing anther; and inducing callus tissue to be subjected to redifferentiation to form the haploid plant. According to the method, the pollen development stage is determined and an external morphological marker is adopted. Since microspore and an anther wall are co-cultured by virtue of a solid culturing medium, low temperature and heat shock pretreatment is performed, callus induction and culturing medium component differentiation are performed and culturing conditions are adjusted, the frequency of callus induction and the differentiating capability of the callus tissue are enhanced. When the method is used for culturing eggplant anther, the callus inductivity reaches over 60%, the emergence rate reaches over 80% and the problem of low inductivity in eggplant anther culturing is solved. By virtue of the method, the haploid plant can be obtained and the emergence rate is high; if the method is applicable to breeding practice, the breeding process can be accelerated and a powerful approach is provided for new variety breeding.

Description

Technical field: [0001] The invention belongs to the field of agriculture, and in particular relates to a method for culturing eggplant anthers to obtain haploid regenerated plants. technical background: [0002] Anther culture is a tissue culture technique for culturing isolated anthers into haploid plants under suitable conditions. It is an effective method for obtaining pure lines at the single-cell level and an important auxiliary means for breeding. Eggplant is one of the important vegetable crops of Solanaceae. It is of great theoretical and practical significance to develop eggplant production and seek varieties with excellent traits such as resistance, high quality, and high yield. The hybrid advantage of eggplant is very significant, which has played an important role in the improvement of varieties; however, the unused germplasm materials of eggplant are decreasing day by day, germplasm resources are scarce, and conventional breeding methods take a long time, heavy...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张燕燕缪其松唐懋华刘叶琼黄忠阳魏猷刚胡静孙雪花
Owner NANJING INST OF VEGETABLE SCI
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