Method for rapid determination of total saponin content in compound ass-hide glue pulp by near infrared spectroscopy

Abstract

The invention discloses a method for rapid determination of the total saponin content in a compound ass-hide glue pulp by near infrared spectroscopy, and belongs to the traditional Chinese medicine research technical field. The method comprises the steps: preparing different concentrations of compound ass-hide glue pulp samples by concentration dilution, and jointly forming a sample set with a finished product sample; acquiring near infrared spectrograms of the sample set, firstly eliminating abnormal samples, and dividing the sample set; then selecting an appropriate spectral wave band and an appropriate pretreatment method to obtain characteristic spectral information of the compound ass-hide glue pulp samples; taking the compound ass-hide glue pulp sample total saponin content tested by a vanillic aldehyde-perchloric acid colorimetric method as a reference value, and building a quantitative calibration model of the relationship between the ass-hide glue pulp near infrared spectrum and the total saponin content by applying a stechiometry technology; and acquiring a near infrared spectrum of a compound ass-hide glue pulp sample with unknown content, and rapidly calculating the total saponin content by utilizing the built quantitative calibration model. The method is beneficial for improving the level of quality control of the compound ass-hide glue pulp, and ensures stability and reliability of the product quality.

Description

technical field [0001] The invention relates to a method for measuring total saponins in compound donkey-hide gelatin pulp, more specifically to a method for quickly measuring total saponins in compound donkey-hide gelatin pulp by using near-infrared spectroscopy. The invention belongs to the technical field of traditional Chinese medicine research. Background technique [0002] Finished drug inspection is the last quality control procedure that drugs must go through before entering the market, and it is directly related to the safety of consumers' medication. At present, the detection methods of traditional Chinese medicines are mostly chromatographic methods, which require complex sample pretreatment before analysis, and the analysis time is also long, and the traditional determination method can only measure one index at a time, prolonging the total consumption of the batch production process. Time. [0003] Compound donkey-hide gelatin pulp is a traditional Chinese medi...

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Problems solved by technology

[0004] In the 2010 edition of "Chinese Pharmacopoeia", the determination of the content of compound donkey-hide gelatin pulp is only one item of total nitrogen, which is not enough to reflect the overall status of the active ingredients in the finished product of compound donkey-hide gelatin pulp, and it is difficult to meet the requirements for content analysis and monitoring of the finished product of compound donkey-hide gelatin pulp

[0006] However, due to the complex composition of Chinese patent medicines, the low content of active ingredients and the serious absorption overlap in their near-infrared spectra, there are still few reports on the near-infrared spectroscopy of traditional Chinese medicine preparations.

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