Method for rapid determination of total saponin content in compound ass-hide glue pulp by near infrared spectroscopy

A technology of compound donkey-hide gelatin and near-infrared spectroscopy, which is applied in the field of determination of total saponins in compound donkey-hide gelatin, can solve the problems of low content of active ingredients, overlapping absorption, difficulty in satisfying the content analysis and monitoring of finished product of compound donkey-hide gelatin, and improve the prediction effect Effect

Active Publication Date: 2014-09-24
SHAN DONG DONG E E JIAO +1
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Problems solved by technology

[0004] In the 2010 edition of "Chinese Pharmacopoeia", the determination of the content of compound donkey-hide gelatin pulp is only one item of total nitrogen, which is not enough to reflect the overall status of the active ingredients in the finished product of compound donkey-hide gelatin pulp, and it is difficult to meet the requirements for content analysis

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  • Method for rapid determination of total saponin content in compound ass-hide glue pulp by near infrared spectroscopy
  • Method for rapid determination of total saponin content in compound ass-hide glue pulp by near infrared spectroscopy
  • Method for rapid determination of total saponin content in compound ass-hide glue pulp by near infrared spectroscopy

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Embodiment 1

[0057] Embodiment 1: Determination of total saponin content in compound donkey-hide gelatin pulp

[0058] 1. Collection of samples:

[0059] Divide 60 batches of finished samples of compound donkey-hide gelatin pulp into two equal parts. Among them, 30 batches of finished products were directly tested for content; the remaining 30 batches of finished products were randomly grouped and combined, and every 5 batches of finished products were combined into one, and a total of 6 samples were obtained, each with a volume of 100mL. The 6 samples were concentrated under reduced pressure at 70°C until the volume was reduced to 50mL, and then diluted step by step with ultrapure water, adding 15mL of ultrapure water each time, and adding water 9 times to the first and third parts, and a total of 18 samples; the remaining 4 samples were added with water 10 times to obtain a total of 40 samples, and the 6 concentrates were operated as above to obtain a total of 58 samples. The diluted s...

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Abstract

The invention discloses a method for rapid determination of the total saponin content in a compound ass-hide glue pulp by near infrared spectroscopy, and belongs to the traditional Chinese medicine research technical field. The method comprises the steps: preparing different concentrations of compound ass-hide glue pulp samples by concentration dilution, and jointly forming a sample set with a finished product sample; acquiring near infrared spectrograms of the sample set, firstly eliminating abnormal samples, and dividing the sample set; then selecting an appropriate spectral wave band and an appropriate pretreatment method to obtain characteristic spectral information of the compound ass-hide glue pulp samples; taking the compound ass-hide glue pulp sample total saponin content tested by a vanillic aldehyde-perchloric acid colorimetric method as a reference value, and building a quantitative calibration model of the relationship between the ass-hide glue pulp near infrared spectrum and the total saponin content by applying a stechiometry technology; and acquiring a near infrared spectrum of a compound ass-hide glue pulp sample with unknown content, and rapidly calculating the total saponin content by utilizing the built quantitative calibration model. The method is beneficial for improving the level of quality control of the compound ass-hide glue pulp, and ensures stability and reliability of the product quality.

Description

technical field [0001] The invention relates to a method for measuring total saponins in compound donkey-hide gelatin pulp, more specifically to a method for quickly measuring total saponins in compound donkey-hide gelatin pulp by using near-infrared spectroscopy. The invention belongs to the technical field of traditional Chinese medicine research. Background technique [0002] Finished drug inspection is the last quality control procedure that drugs must go through before entering the market, and it is directly related to the safety of consumers' medication. At present, the detection methods of traditional Chinese medicines are mostly chromatographic methods, which require complex sample pretreatment before analysis, and the analysis time is also long, and the traditional determination method can only measure one index at a time, prolonging the total consumption of the batch production process. Time. [0003] Compound donkey-hide gelatin pulp is a traditional Chinese medi...

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Application Information

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IPC IPC(8): G01N21/359G01N21/3577
Inventor 秦玉峰尤金花田守生周祥山瞿海斌李文龙韩海帆张淹张路
Owner SHAN DONG DONG E E JIAO
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