Lactic acid bacteria culture medium for producing lactase by fermentation

A culture medium and lactic acid bacteria technology, applied in the biological field, can solve the problems of polluting the ecological environment, waste of whey nutrients, etc., and achieve the effects of reducing pollution, improving the activity of lactase, and increasing the yield of lactase.

Active Publication Date: 2016-09-28
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, at present, a large amount of whey is directly discharged as wastewater, seriously polluting the ecological environment, and a large amount of nutrients contained in whey are wasted

Method used

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  • Lactic acid bacteria culture medium for producing lactase by fermentation
  • Lactic acid bacteria culture medium for producing lactase by fermentation
  • Lactic acid bacteria culture medium for producing lactase by fermentation

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Experimental program
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Embodiment Construction

[0028] The present invention is further illustrated below by means of examples, but the present invention is not limited to the scope of the examples.

[0029] The lactic acid bacterium of the present invention selects Lactobacilli (Lactobacilli).

[0030] The present invention inoculates the cultured seed solution into 100mL different fermentation medium (capacity 250mL Erlenmeyer flask) with an inoculum size of 1% (v / v), leaves it standing at 35°C for 24h, and measures the activity of β-galactosidase. And according to the size of the enzyme activity to determine the optimum concentration of each component of the medium.

[0031] 1. Add different concentrations of whey powder (final concentrations are 10g / L, 15g / L, 20g / L, 25g / L, 30g / L respectively) in the culture medium, and the remaining components and contents remain unchanged. Incubate at 35°C for 24 hours, and measure lactase activity.

[0032] 2. On the basis of the above experiments, the other components of the whey p...

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Abstract

The invention relates to a lactic acid bacteria culture medium for fermenting and producing lactase. The culture medium components include whey powder, yeast extract powder, NH 4 h 2 PO 4 , Inorganic salts and vitamins are also included in the culture medium. The culture medium of the invention can grow lactic acid bacteria well and produce high-activity lactase; at the same time, compared with the culture medium before optimization, the production cost of the culture medium of the invention is greatly reduced by up to 50%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a lactic acid bacteria culture medium, in particular to a lactic acid bacteria culture medium for fermenting and producing lactase. Background technique [0002] Lactase can catalyze the hydrolysis of lactose into galactose and glucose, and also has the function of transgalactoside. Lactase is widely used. In the dairy industry, lactase is often used to produce low-lactose dairy products and galacto-oligosaccharides. Using lactase to hydrolyze lactose can also effectively reduce the problems of lactose crystallization and whey precipitation in frozen concentrated dairy products. Adding a certain amount of lactase during food processing can also improve the sweetness, solubility and flavor of food. In terms of environmental protection, lactase can decompose lactose in whey and slow down the pollution of water caused by the high biological oxygen demand after whey is discharged. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/38C12R1/225
Inventor 杨洪江李梦菲朱慧明
Owner TIANJIN UNIV OF SCI & TECH
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