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Lactic acid bacteria culture medium for production of conjugated linoleic acid

A technology of conjugated linoleic acid and culture medium, which is applied in the field of lactic acid bacteria culture medium for producing conjugated linoleic acid, can solve the problems of high price, complex composition, and complicated production, and achieves the effect of reducing production cost and improving vitality

Inactive Publication Date: 2015-12-02
TIANJIN MODERN VOCATIONAL TECH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nutrient substance of this medium is not only complicated in composition, expensive, but also cumbersome to make, does not have the ability of industrial production, and the yield of linoleic acid produced by the strain is not high

Method used

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  • Lactic acid bacteria culture medium for production of conjugated linoleic acid
  • Lactic acid bacteria culture medium for production of conjugated linoleic acid
  • Lactic acid bacteria culture medium for production of conjugated linoleic acid

Examples

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Embodiment 1

[0029] A lactic acid bacteria culture medium for producing conjugated linoleic acid in this embodiment includes: 6-14% of Jerusalem artichoke juice, 3% of glucose, 3% of yeast extract, 4% of sodium acetate, and 2% of potassium dihydrogen phosphate. %, magnesium sulfate 0.05%, manganese sulfate 0.05%, the above-mentioned components are percentages by mass, Tween-801ml / L, and the pH value of the culture medium is 6.8.

[0030] The method for cultivating lactic acid bacteria to produce conjugated linoleic acid with the above-mentioned medium comprises the following steps in turn:

[0031] (1) Preparation of Jerusalem artichoke dipping juice: Accurately weigh 1000g of Jerusalem artichoke, pre-cook it, then add water to extract the juice, add water to make up to 1000ml, sterilize at 115°C for 10 minutes, cool for later use.

[0032] (2) Culture medium preparation: according to the mass percentage, accurately weigh 6-14% of Jerusalem artichoke juice, 3% of glucose, 3% of yeast extra...

Embodiment 2

[0038] A lactic acid bacteria culture medium for producing conjugated linoleic acid in this embodiment includes: 10% of Jerusalem artichoke juice, 0.5-3.5% of glucose, 3% of yeast extract, 4% of sodium acetate, and 2% of potassium dihydrogen phosphate , magnesium sulfate 0.05%, manganese sulfate 0.05%, the contents of the above components are percentages, Tween-801ml / L, and the pH value of the culture medium is 6.8.

[0039] The method for cultivating lactic acid bacteria to produce conjugated linoleic acid with the above-mentioned medium comprises the following steps in turn:

[0040] (1) Preparation of Jerusalem artichoke dipping juice: Accurately weigh 1000g of Jerusalem artichoke, pre-cook it, then add water to extract the juice, add water to make up to 1000ml, sterilize at 115°C for 10 minutes, cool for later use.

[0041](2) Culture medium preparation: according to the mass percentage, accurately weigh 10% of Jerusalem artichoke juice, 0.5-3.5% of glucose, 3% of yeast ex...

Embodiment 3

[0047] A lactic acid bacteria culture medium for producing conjugated linoleic acid in this embodiment comprises: 10% of Jerusalem artichoke juice, 2% of glucose, 2-6% of yeast extract, 4% of sodium acetate, and 2% of potassium dihydrogen phosphate. %, magnesium sulfate 0.05%, manganese sulfate 0.05%, Tween-801ml / L, and the pH value of the culture medium is 6.8.

[0048] The method for cultivating lactic acid bacteria to produce conjugated linoleic acid with the above-mentioned medium comprises the following steps in turn:

[0049] (1) Preparation of Jerusalem artichoke dipping juice: Accurately weigh 1000g of Jerusalem artichoke, pre-cook it, then add water to extract the juice, add water to make up to 1000ml, sterilize at 115°C for 10 minutes, cool for later use.

[0050] (2) Culture medium preparation: accurately weigh 10% of Jerusalem artichoke juice, 2% of glucose, 2-6% of yeast extract, 4% of sodium acetate, 2% of dipotassium hydrogen phosphate, 0.05% of magnesium sulfat...

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Abstract

The invention provides a lactic acid bacteria culture medium for production of conjugated linoleic acid. The lactic acid bacteria culture medium comprises, by mass, 6-14% of jerusalem artichoke extractive juice, 0.5-4% of glucose, 1-6% of yeast extracts, 2-6% of sodium acetate, 2% of dipotassium phosphate, 0.05% of magnesium sulfate and 0.05% of manganese sulfate, 1ml / L of Tween-80 is used, water serves as a solvent and the pH (potential of hydrogen) value of the culture medium is 6.8. The lactic acid bacteria culture medium has the advantages that nutrient substances such as beef extracts, peptone and ammonium citrate in MRS culture media are replaced by jerusalem artichoke, so that basic nutritional requirements for lactic acid bacteria growth can be met, and activity of linoleic acid in lactobacillus is improved; moreover, the jerusalem artichoke and inorganic nitrogen have extensive sources and are economical and practical, so that production cost is reduced.

Description

technical field [0001] The invention relates to a lactic acid bacteria culture medium, in particular to a lactic acid bacteria culture medium for producing conjugated linoleic acid. Background technique [0002] Linoleic acid is a general term for a group of geometric and positional isomers with conjugated double bonds of Linoleic Acid (LA), an essential fatty acid for the human body. In recent years, conjugated linoleic acid, as a functional oil, has attracted more and more attention, and has become a research hotspot in the fields of pharmacy, chemistry and food. Conjugated linoleic acid has the functions of anti-cancer, anti-oxidation, anti-atherosclerosis, lowering cholesterol, and promoting growth. It has been widely used in functional food, medicine, health care products, cosmetics and other fields. There are still relatively few reports on the application of conjugated linoleic acid in clinical medicine, but its functions of weight loss, anti-cancer, and anti-athero...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/25
Inventor 陈珊王立晖殷海松潘志恒汤卫华张乐黄艳玲程秀玮龙尾吕春晖苑鹏刘鑫龙高媛王玉姝高芦宝贾晓鹏
Owner TIANJIN MODERN VOCATIONAL TECH COLLEGE
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