A kind of feed additive and preparation method thereof
A technology of feed additives and raw materials, applied in the field of high-efficiency feed additives and its preparation, can solve problems such as difficult to realize industrialization, standardization, influence on feed nutrition ratio, increase product cost, etc., to enhance cellular immunity and humoral immunity, improve daily Weight gain and feed conversion ratio, the effect of saving usage
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preparation example Construction
[0037] (1) Preparation of bacterial suspension
[0038] Inoculate the single colony of Li-2013 grown after streaking on the plate into the seed medium, culture at 100r / min, 40°C for 12h, take 1mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend in 9mL of physiological in salt water.
[0039] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis
[0040] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows colonies, p...
Embodiment 1
[0057] Embodiment 1: preparation method is the same as example 3
[0058] A feed additive comprises the following raw materials in parts by weight: 27 parts of astragalus extract; 15 parts of Codonopsis pilosula extract; 16 parts of Bupleuri extract; 20 parts of Bacillus subtilis culture.
Embodiment 2
[0060] Preparation of Bacillus subtilis culture:
[0061] Obtain Bacillus subtilis fermentation broth by step-by-step expansion culture of slant strains;
[0062] (1) Primary seed culture: Inoculate the slant strain of Bacillus subtilis into a 500-ml shake flask, with a medium volume of 100 ml, a rotary shaker at 180 rpm, a culture temperature of 40°C, and a culture time of 12 hours;
[0063] (2) secondary seed cultivation: the primary seed is inserted into a 500 milliliter secondary seed shake flask according to the inoculum size of 10%, and the cultivation condition is the same as that of the primary seed;
[0064] (3) Tertiary seed cultivation: Inoculate the secondary seeds with 10% inoculum amount into a 5000 ml tertiary seed shake flask, the medium loading capacity is 1000 ml, the rotary shaker is 100 rpm, and the cultivation temperature is 40°C. Time 12 hours;
[0065] (4) First-level seed tank cultivation: put the third-level seeds into the first-level seed tank with ...
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