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Extraction of Microalgae

A technology of microalgae and microwave radiation, applied to the preparation method of peptides, peptides, sugar derivatives, etc., can solve the problems of time-consuming and increased extraction process costs

Inactive Publication Date: 2016-10-19
THE FLINDERS UNIV OF SOUTH AUSTRALIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Drying and / or solvent extraction steps take time and add cost to the extraction process

Method used

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  • Extraction of Microalgae
  • Extraction of Microalgae
  • Extraction of Microalgae

Examples

Experimental program
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Effect test

example

[0133] The microalgal biomass was obtained from the marine microalga Nannochloropsis. Nannochloropsis sp. were grown in 3000L outdoor track tanks filled with 20ppt seawater and supplemented with F / 2 medium. pH value generally use 1-5% CO 2 Controlled at 7.9 to 8.2. In outdoor conditions, temperature and lighting were not controlled.

[0134] Microalgae can also be cultured in photobioreactor systems, where many parameters including temperature and light intensity can be controlled under sterile conditions.

example 1

[0135] Example 1 - Microwave Extraction of Dried Microalgal Biomass under Acidic and Basic Conditions

[0136] Material

[0137] Lot 29 of dry microalgal biomass was obtained from the South Australian Research and Development Institute (SARDI).

[0138] equipment

[0139] Microwave extraction was performed at the Start Synth Microwave Synthesis Experimental Station in Milestone.

[0140] program

[0141] Dry biomass (2.5 g) was added to a 50 mL round flat bottom flask. Add 25 mL of 0.5M HO to the sample 2 SO 4 solution or 25 mL of 0.5M NaOH. Next, the samples were incubated at room temperature for about 1 hour, followed by microwave irradiation at 100°C for 2, 5, 10 or 30 minutes.

[0142] Samples were then centrifuged at 10,000g for 10 minutes and the top (lipid) and middle (carbohydrate and protein) layers were collected separately.

[0143] Lipid extracts were analyzed gravimetrically, carbohydrate extracts were analyzed by DNS method, and protein extracts were anal...

example 2

[0173] Example 2 - Scale-up of extraction under selected conditions

[0174] To recover most of the carbohydrates and proteins during microwave extraction, acidic to basic (acid-basic-basic-acid-acid-basic) repeat conditions were chosen. according to Figure 9 and 10 , the total carbohydrate content extracted from the dry microalgal biomass by six extractions is at most 5%, and the protein content extracted from the dry microalgal biomass by six extractions is at most 10%. In addition, according to Figure 9 , most of the extracted carbohydrates could be extracted by the first acidic extraction, and only a very small amount of extracted carbohydrates were provided by the second and third extractions. For protein, the first acidic extraction accounted for about 25% of the total extracted protein, and the second and third extractions accounted for about 30% and 20% of the total extracted protein, respectively. Thus, acidic to alkaline conditions not only maximize carbohydrat...

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Abstract

The present invention provides a method of extracting a lipid-containing product from microalgal biomass, the method comprising: (i) treating an aqueous mixture comprising microalgal biomass with microwave radiation, and (ii) recovering from the treated microalgal biomass Substance recovery lipid-containing product.

Description

[0001] priority document [0002] This application claims priority to Australian Provisional Patent Application No. 2011904343, entitled "MICROALGAL EXTRACTION", filed 20 October 2011, the contents of which are hereby incorporated by reference in their entirety. technical field [0003] The present invention relates to methods for extracting material from microalgal biomass. Background technique [0004] In the long run, fossil fuels cannot be used as a transportation fuel. This, combined with the negative environmental impact of fossil fuel combustion, has led to research into renewable energy sources that can provide alternatives to non-renewable fossil fuels. Biofuels are one such renewable energy source. Biofuels are fuels made from renewable organic sources or raw materials. The term generally refers to transportation fuels and includes ethanol and biodiesel. As of now, the main source of oil used to make biodiesel is food crops. However, the use of food crops als...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/12C11B1/10C11B1/02C11B1/04
CPCA23D9/02C11B1/04C11B1/08C11B1/12C11B1/10Y02E50/10C07K1/145C07H1/08
Inventor 张卫宿鹏
Owner THE FLINDERS UNIV OF SOUTH AUSTRALIA
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