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A kind of in vitro culture method of Shennong chrysanthemum
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A technology for in vitro culture of Shennong fragrant chrysanthemum is applied in the field of in vitro culture of Shennong fragrant chrysanthemum, which can solve the problems of the optimization system of strong seedlings, the limitation of material sources, and the thinness of tissue culture seedlings, so as to achieve sufficient materials and improve the reproduction coefficient. , the effect of convenient sampling
Inactive Publication Date: 2016-08-24
CHINA THREE GORGES UNIV +1
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Using the buds of Shennongxiangchrysanthemum as explants, the callus of Shennongxiangchrysanthemum buds was successfully induced and regenerated plants were obtained, but limited by the source of materials
There is also an in vitro regeneration system of Shennong chrysanthemum induced by in vitro germination of axillary buds to induce adventitious buds, but the problem of thin and leggy tissue culture seedlings has not been effectively solved, and there are also problems in the optimization system of its strong seedlings
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Embodiment 1
[0032] Embodiment 1, the in vitro culture of Shennong Fragrant Chrysanthemum
[0033] MS basal medium: It can be prepared by referring to the literature (Murashige T, Skoog F.A revised medium for rapid groove and bioassays with tobacco tissue cultures. Physiol. Plant, 1962, 15:473-497).
[0034] The in vitro culture method of Shennong Xiangchrysanthemum of the present invention comprises the following steps:
[0035] (1) Sterilization of stem explants
[0036] Remove the leaves from the young stems of Shennong Chrysanthemum chrysanthemum, soak them for 20-40 minutes in a cleaning solution with a concentration of 4-8 drops per 100ml (Diaopai cleaning solution produced by Naaisi Group Co., Ltd.), then scrub with water, and then wash them with water. Rinse for 1-2h, then place it on a clean bench and soak it in 75% alcohol for 10-30s, then sterilize it with 0.1% HgCl2 solution containing 2-4 drops of Tween-40 per 100ml for 15min, use sterile Wash with water 4-6 times, 1-3min ea...
Embodiment 2
[0049] Embodiment 2, the in vitro culture of Shennong Fragrant Chrysanthemum
[0050] MS basal medium: It can be prepared by referring to the literature (Murashige T, Skoog F.A revised medium for rapid groove and bioassays with tobacco tissue cultures. Physiol. Plant, 1962, 15:473-497).
[0051] The in vitro culture method of Shennong Xiangchrysanthemum of the present invention comprises the following steps:
[0052] (1) Sterilization of stem explants
[0053] Remove the leaves from the young stems of Shennong Chrysanthemum chrysanthemum, soak them for 20-40 minutes in a cleaning solution with a concentration of 4-8 drops per 100ml (Diaopai cleaning solution produced by Naaisi Group Co., Ltd.), then scrub with water, and then wash them with water. Rinse for 1-2h, then place it on a clean bench and soak it in 75% alcohol for 10-30s, then sterilize it with 0.1% HgCl2 solution containing 2-4 drops of Tween-40 per 100ml for 15min, use sterile Wash with water 4-6 times, 1-3min ea...
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Abstract
The invention discloses an in vitro culture method of Shennong Fragrant Chrysanthemum, which uses sterilized young stems with buds as explants, induces clustered buds, cultivates strong seedlings, takes root, hardens seedlings and transplants Obtain Shennong Xiangchrysanthemum regenerated plants, wherein: the young stems with buds are cleaned before sterilization, soaked in a detergent solution with a concentration of 0.2-0.8% for 20-40min, then brushed and rinsed with water for 1-2h ; During the cultivation of strong seedlings and rooting culture, the wild ancient grass extract and activated carbon were added in the culture medium; The method of the present invention can induce a large number of clustered buds of Shennongxiangchrysanthemum at a high frequency, and the survival rate of regenerated plants is high, which can reach 100%. It has laid a good foundation and has broad application prospects.
Description
technical field [0001] The present invention relates to the propagation technology of Shennong chrysanthemum, more specifically to a method for in vitro cultivation of Shennong chrysanthemum. Background technique [0002] Shennongxiangju (Dendranthema indicum (L.) Des Moul.var.aromaticum Q.H.Liuet S.F.Zhang Var.Nov.) is a new resource plant first discovered and named by Mr. Liu Qihong of Wuhan Institute of Botany in Shennongjia in 1982. It is a perennial herb. The leaves are small and thick, dark green, the upper veins are obviously raised, the lower surface is covered with sunken tiny glands, and the flower heads are small, usually no more than 1.5 cm in diameter. The main difference from the wild chrysanthemum is that the flowers, leaves and roots all have special Strong aroma. Shennong Fragrant Chrysanthemum is concentrated in the sunny and open hillsides and roadsides at an altitude of 2600-2700 meters in Shennongjia. Moul.). Folks in the mountainous areas of western ...
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