Copper ion spectrum probe as well as preparation method and application thereof

A copper ion and spectral technology, applied in the field of spectral probes, can solve problems such as environmental pollution and endangering human health, and achieve high sensitivity, accurate results, and good selectivity

Inactive Publication Date: 2014-10-29
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the excessive concentration of copper ions in soil or water can easily cause environmental pollution and endanger human health.

Method used

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  • Copper ion spectrum probe as well as preparation method and application thereof
  • Copper ion spectrum probe as well as preparation method and application thereof
  • Copper ion spectrum probe as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: The specific synthesis steps and single crystal cultivation of CSFH are as follows:

[0023] Dissolve 0.5mmol fluorescein hydrazide in 15mL of absolute ethanol in the reactor, then add 0.5mmol of 5-Cl-salicylaldehyde solution dissolved in 10mL of absolute ethanol, stir and reflux for 2h, cool, filter, collect the filtrate and let it slowly Light yellow single crystals were precipitated by volatilization, and the precipitate collected by filtration was recrystallized to obtain CSFH solid powder with a yield of 70%.

[0024] Characterization of CSFH: Melting point: >270°C. Elemental analysis (calcd.%): C, 66.87; H, 3.51; N, 5.78; O, 16.51; Cl, ​​7.33; found: C, 65.86; 1 H NMR (300MHz, 25°C, DMSO-d 6 ): δ10.412(s,1H), 9.937(bs,2H), 9.079(s,N=C-H,1H),7.914(m,1H),7.617(t,2H),7.375(d,1H), 7.202 (d, 1H), 7.133 (d, 1H), 6.780 (d, 1H), 6.642 (bs, 2H), 6.478 (d, 4H). Crystal data for C 27 h 17 N 2 o 5 Cl: crystal size: 0.35×0.33×0.32, monoclinic, spacegroup C2 / ...

Embodiment 2

[0025] Example 2: Selective recognition of copper ions by CSFH

[0026] Mix PBS (pH=9) buffer solution with 1mmol·L -1 CSFH ethanol solution was added to 18 clean UV cuvettes at a ratio of 100:1, one of which was blank, and 1 times the equivalent of Ca was added to the other 2+ , Mg 2+ , Al 3+ , Ba 2+ , Fe 2+ , Hg 2+ , Fe 3+ ,Co 2+ , Ni 2+ , Mn 2+ , Cr 3+ , Cd 2+ , Pb 2+ , Cr 6+ , Cu 2+ , Ag + and Zn 2+ After that, their absorption spectra were measured respectively, such as figure 2 As shown, CSFH has a unique selectivity for copper ions.

Embodiment 3

[0027] Embodiment 3: Other common metal ions interfere with detecting copper ions

[0028] Mix PBS (pH=9) buffer solution with 1mmol·L -1 CSFH ethanol solution was added to 17 clean UV cuvettes at a ratio of 100:1, and 1 times the equivalent of Cu was added to each 2+ , and then add 3 times the equivalent of other metal ions Ca 2+ , Mg 2+ , Al 3+ , Ba 2+ , Fe 2+ , Hg 2+ , Fe 3+ ,Co 2+ , Ni 2+ , Mn 2+ , Cr 3+ , Cd 2+ , Pb 2+ , Cr 6+ , Ag + and Zn 2+ After that, the absorbance value at 495nm was measured respectively, and the absorbance value of the solution when a single copper ion existed was A 0 , the absorbance value of the solution where copper ions coexist with other common metal ions is A x , with A x / A 0 Draw a histogram of UV absorption corresponding to different metal ions for the ordinate, such as image 3 shown. Other common metal ions do not interfere with the system for the determination of copper ions.

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Abstract

The invention discloses a copper ion spectrum probe as well as a preparation method and an application thereof. A synthetic method for the copper ion spectrum probe comprises the step of enabling 5-R-salicylaldehyde to react with fluorescein hydrazide to obtain the probe. The probe has a good complexing action with copper ions, so that the spectral absorption is strengthened. In the presence of copper ions, quick change of the color of a solution from colorlessness to yellow can be observed with naked eyes, and a new absorption peak appears at 495nm of the ultraviolet visible absorption spectrum. The stoichiometric ratio of the probe to the copper ions is 1: 1, and the measurement of the copper ions is not disturbed by other common metal ions. The copper ion spectrum probe is easy to synthesize, simple in operation, low in environmental pollution, easy to produce and the like, and has very high selectivity and sensitivity on the copper ions. Therefore, the probe can be used for simply, sensitively and quickly detecting the copper ions, and has practical value in a biosystem and environmental monitoring.

Description

technical field [0001] The invention relates to a spectral probe, in particular to a copper ion spectral probe and its preparation method and application. Background technique [0002] As one of the indispensable trace elements for the human body, copper ions contribute to the synthesis of hemoglobin in the organism and the maintenance of the activity of various metalloenzymes. Changes in the content of copper ions in cells can cause many serious diseases, such as: hepatolenticular degeneration disease, Alzheimer's disease, muscular dystrophy, prion disease, etc. In addition, excessive concentrations of copper ions in soil or water can easily cause environmental pollution and endanger human health. Designing a synthetically simple, sensitive, fast, and accurate spectral probe for the detection of copper ions is of great significance to the fields of biological systems and the environment. Contents of the invention [0003] The object of the invention is to provide a copp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/64
Inventor 武鑫董川张建刚弓晓娟
Owner SHANXI UNIV
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