Test paper strip using colloidal gold immunochromatographic technology for quantitative determination of serum pepsinogen and preparation method and application thereof

A pepsinogen and quantitative detection technology, which is applied in the field of rapid screening and efficacy evaluation of gastric diseases or early gastric cancer, can solve the problems of long detection time, high detection cost, unsuitable for single test, etc., and achieves the effect of convenient operation.

Active Publication Date: 2014-11-12
BEIJING MOKOBIO LIFE SCI CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] One of the technical problems to be solved by the present invention is to overcome the shortcomings of the conventional pepsinogen detection method, which is not suitable for single-person testing, the detection time is long, and the detection cost is high, and to provide a fast, simple, accurate, and low-cost method for quantitative detection of PG. method

Method used

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  • Test paper strip using colloidal gold immunochromatographic technology for quantitative determination of serum pepsinogen and preparation method and application thereof
  • Test paper strip using colloidal gold immunochromatographic technology for quantitative determination of serum pepsinogen and preparation method and application thereof
  • Test paper strip using colloidal gold immunochromatographic technology for quantitative determination of serum pepsinogen and preparation method and application thereof

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Embodiment 1

[0041] Embodiment 1 Preparation of test strips for the quantitative detection of serum pepsinogen using colloidal gold immunochromatography technology of the present invention

[0042] The preparation flow chart is as figure 1 shown.

[0043] 1. Preparation of human pepsin antigen I or protease antigen II natural protein

[0044] Collect surgically resected normal human gastric tissue, rinse with phosphate buffer, separate gastric mucosa, blot dry, weigh, add phosphate buffer, mash and homogenate, centrifuge, collect supernatant, precipitate phosphate buffer weight suspended, centrifuged again, and combined the supernatant to obtain the pepsinogen crude extract;

[0045] Add the pepsinogen crude extract to the equilibrated DEAE-52 chromatographic column, elute the chromatographic column with PBS buffer until the absorbance value is 0 at 280nm, continue to elute the chromatographic column with PBS buffer, and combine the eluted Peak off to obtain active pepsinogen;

[0046]...

Embodiment 2

[0057] The use and result judgment of the colloidal gold test strip of the present invention

[0058] Dilute the prepared human pepsin antigen I and human pepsin antigen II to 22, 46, 63, 100, 200ug / L and 2.8, 4.8, 11.5, 25, 43ug / L solutions respectively, as standard solution, and prepare at the same time blank solution. Take 60μl standard and sample to be tested (30μl sample + 30μl sample diluent) and drop them into the test strip sample groove. After 20 minutes, put the color-developed standard and the test strip of the sample to be tested into the immunochromatography reading In the instrument (Beijing Meikang Biotechnology Research Center Co., Ltd.), read the color value of the T line and record it. The schematic diagram of the color development result is as follows image 3 Shown; Bring the color value of the sample to be tested into the standard curve to obtain the concentration result of pepsinogen I or pepsinogen II.

[0059] The pepsinogen colloidal gold immunochrom...

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Abstract

The invention discloses a test paper strip using colloidal gold immunochromatographic technology for quantitative determination of serum pepsinogen and a preparation method and application thereof. A kit comprises the test paper strip respectively used for detection of pepsinogen I and pepsinogen II, the test paper strip orderly comprises a sample pad, a gold labeled pad, a cellulose nitrate film and a water absorbent pad according to the order of connection, and also includes a plastic bottom lining located below, the role of the plastic bottom lining is to provide a platform for assembling, the cellulose nitrate film is provided with a pepsinogen I or pepsinogen II spraying quality control line, and a pepsinogen I or pepsinogen II second antibody spraying test line, the gold labeled pad is coated with a colloidal gold labeled pepsinogen I or pepsinogen II monoclonal antibody, and the sample pad provides a to-be-tested sample adding position. The kit can be used for direct quantitative detection of the pepsinogen without need of professional training, is convenient and fast in operation, and suitable for promotion and application in primary hospitals and medical centers.

Description

technical field [0001] The present invention relates to a reagent for screening gastric diseases, in particular to a gastric-related disease that uses colloidal gold immunochromatography technology and quantitatively detects the contents of pepsinogen I and pepsinogen II in human serum to screen and evaluate the therapeutic effect. A test strip for diseases and early gastric cancer belongs to the field of rapid screening and curative effect evaluation for gastric diseases or early gastric cancer. Background technique [0002] Gastric cancer is the second leading disease with high risk of mortality in the world, which seriously threatens human health. Gastric cancer is one of the most common malignant tumors, and its death rate ranks among the top among all kinds of malignant tumors. There are 934,000 new cases of gastric cancer every year in the world, and 42% (nearly 400,000) of the new cases are in my country. The prevalence of gastric cancer in my country and The death ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531G01N33/558
CPCG01N33/558G01N33/573G01N33/57446
Inventor 金鑫曾滨
Owner BEIJING MOKOBIO LIFE SCI CO LTD
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