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Chloroperoxidase catalyzed oxidation of hair decolorizing agent and method and application of decolorization

A chloroperoxidase, catalytic oxidation technology, applied in the field of decolorization treatment, to achieve the effect of convenient operation, simple treatment process and mild reaction conditions

Inactive Publication Date: 2017-08-25
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CPO has a very wide range of oxidation activities, both heme peroxidase (one-electron and two-electron peroxidation, organic halogenation), catalase (hydrogen peroxide disproportionation, monohydric alcohol oxidation) and cytochrome P-450 ( The catalytic characteristics of various enzymes such as dealkylation, olefinic bond epoxidation, sulfonation oxidation, etc.) are currently considered to be the most widely used enzymes in the peroxidase family, but peroxidase is used as a catalyst to oxidize black Hair decolorization has not been reported yet

Method used

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  • Chloroperoxidase catalyzed oxidation of hair decolorizing agent and method and application of decolorization
  • Chloroperoxidase catalyzed oxidation of hair decolorizing agent and method and application of decolorization

Examples

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Effect test

Embodiment 1

[0032] Completely immerse the 20 mg black hair sample to be decolorized in the phosphate buffer solution containing sodium chloride, adjust the pH value of the solution to 2.75, soak for one hour, and then add 150 μL of the substance at a concentration of 0.25 mmol L -1 Chloroperoxidase, and then add 30% H 2 o 2 Aqueous solution, wherein the concentration of chloroperoxidase in the mixed solution is 1.25×10 -2 mmol / L, and chloroperoxidase, H 2 o 2 The molar ratio to sodium chloride is 3.75×10 -5 : 3.95: 5.2, after mixing evenly, decolorize the black hair by catalytic oxidation at room temperature for 24 hours, observe that the color of the black hair sample is yellowish brown, and the decolorization effect is obvious.

Embodiment 2

[0034] Completely immerse the 20mg black hair sample to be decolorized in the phosphate buffer solution containing sodium chloride, adjust the pH value of the solution to 3, soak for 0.5 hours, and then add the substance at a concentration of 0.25mmol L -1 Chloroperoxidase, and then add 30% H 2 o 2 Aqueous solution, wherein the concentration of chloroperoxidase in the mixed solution is 0.8×10 -2 mmol / L, and chloroperoxidase, H 2 o 2 The molar ratio to sodium chloride is 2.4×10 -5 :3:2.5, after mixing evenly, decolorize the black hair by catalytic oxidation at room temperature for 15 hours, observe that the color of the black hair sample is yellowish brown, and the decolorization effect is obvious.

Embodiment 3

[0036] Completely immerse the 20mg black animal hair sample to be decolorized in the phosphate buffer solution containing sodium chloride, adjust the pH value of the solution to 4, soak for 0.7 hours, and then add the substance at a concentration of 0.25mmol L -1 Chloroperoxidase, and then add 30% H 2 o 2 Aqueous solution, wherein the concentration of chloroperoxidase in the mixed solution is 1.0×10 -2 mmol / L, and chloroperoxidase, H 2 o 2 The molar ratio to sodium chloride is 3.0×10 -5 : 2.9: 4, after mixing evenly, decolorize the black animal hair by catalytic oxidation at room temperature for 16 hours, the color of the black animal hair sample is observed to be yellowish brown, and the decolorization effect is obvious.

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Abstract

The invention discloses a chloroperoxidase catalyzed oxidation hair decolorization agent and a decolorization method and application thereof. The hair decolorization agent of the invention is based on a biological enzyme catalyzed oxidation system, and a phosphate buffer solution containing chloride ions is added to a black hair sample to be decolorized , adjust the pH of the solution to 2.75-5, soak for one hour, then add chloroperoxidase, then use H2O2 as an oxidant, add 30% H2O2 solution to the mixed solution, mix evenly, and catalyze the oxidation of black hair at room temperature to decolorize. The system of the present invention has simple composition, convenient operation, environmental protection, less dosage of chloroperoxidase and H2O2, mild reaction conditions, can destroy melanin in hair, and has obvious decolorization effect, and black hair samples turn yellowish brown in 24 hours.

Description

technical field [0001] The invention belongs to the technical field of decolorization treatment, and in particular relates to a chloroperoxidase catalyzed oxidation of a hair decolorization agent and a decolorization method and application thereof. Background technique [0002] With the advancement of science and technology and the improvement of living standards, people's ideas are also changing, and more people begin to pay attention to their own image. People's pursuit of beauty is getting higher and higher, and they are no longer satisfied with a single black human hair, hoping to change their image by changing the color of their hair. [0003] Hair is a kind of natural protein fiber. The natural color in hair is formed by itself during the growth process. These colors are mainly determined by melanin in the human body. Generally speaking, the higher the melanin content, the darker the hair color. Some strong oxidants can destroy melanin and make it decolorize. Usually...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D06L4/40D06L4/13D06L4/22D06L4/12
Inventor 蒋育澄崔茹胡满成李淑妮翟全国
Owner SHAANXI NORMAL UNIV
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