Method for soybean oil decoloring by beta-carotene-15,15'-monooxygenase

A monooxygenase, carotene technology, applied in the direction of oil/fat refining, fat production, etc., can solve the problems of oil quality influence, oil residual decolorizer smell and so on

Inactive Publication Date: 2016-02-03
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

However, physical decolorization will lead to the residual decolorizing agent and the smell of decolorizing agent in the decolorized oil,...

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 2

[0010] Embodiment 2: The difference between this embodiment and the specific embodiment is that the amount of the extracted enzyme solution added in step 2 is 8, 10, 12 mL. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0011] Embodiment 3: The difference between this embodiment and the specific embodiment is that the concentration of cholate added in step 2 is 0.3, 0.5, 0.7 mmol / L, and the addition amount is 1.0, 1.5, 2.0 mL. Other steps are the same as in the first embodiment.

specific Embodiment approach 4

[0012] Embodiment 4: The difference between this embodiment and the specific embodiment is that the decolorization temperature in step 2 is 30, 35, 40° C., and the stirring speed is 40, 50, 60 / min. Other steps are the same as in the first embodiment.

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Abstract

The invention aims at providing a method for soybean oil decoloring by beta-carotene-15,15'-monooxygenase. According to the method, firstly, beta-carotene-15,15'-monooxygenase is extracted from the chicken intestine mucous membrane; a detection result shows that the protein concentration of extracts is 5.87mg/mL; the decolorization ratio of the third-class soybean oil is used as the index for studying the effect of the beta-carotene-15,15'-monooxygenase on the beta-carotene in the three-class soybean oil under the conditions of different enzyme extraction liquid adding quantity, sodium cholate concentration, reaction time and stirring speed. The result proves that when the beta-carotene-15,15'-monooxygenase is used for decoloring the three-class soybean oil, the finally obtained decoloring rate of the enzyme process decoloring is 32.74 percent.

Description

Technical field: [0001] The invention belongs to the field of oil refining and relates to a method for decolorizing soybean oil by using beta-carotene-15,15'-monooxygenase. technical background: [0002] There are three main pigment substances in soybean oil: organic pigments of known composition, organic decomposition products, and pigments due to oxidation and colorless precursors. β-carotene is the main target of decolorization. The carotenoids in the oil make the oil red and absorb light in the wavelength range of 400-500nm. [0003] In the oil refining process, the decolorization process is not simply to remove all the pigments in the oil, but to improve the color of the oil and provide qualified raw material oil for oil deodorization, so oil decolorization is one of the key steps in the oil refining process . The decolorization of soybean oil usually uses physical method decolorization, that is, adsorption decolorization. Commonly used decolorizers include activated...

Claims

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Application Information

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IPC IPC(8): C11B3/00
Inventor 于殿宇潘明喆李俊江连洲王立琦杜华楠周爽张旭任悦
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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