Micro-fluidic- technique-based living cell culture device

A microfluidic technology and cell culture technology, which is applied in the field of living cell culture devices, can solve the problems of high technical requirements for operators, cumbersome and complicated technical operations, and long residence time of embryos, so as to avoid long residence time and overcome tedious procedures. The effect of technical operation and reasonable structure of the device

Active Publication Date: 2014-12-03
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, this field mainly adopts the traditional manual operation method, which needs to transfer the target into multiple different petri dishes at different periods.

Method used

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  • Micro-fluidic- technique-based living cell culture device
  • Micro-fluidic- technique-based living cell culture device
  • Micro-fluidic- technique-based living cell culture device

Examples

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Example Embodiment

[0032] Such as Figure 1~16 As shown, the living cell culture device based on microfluidic technology of the present invention includes a microfluidic culture chip set 1, a rotary joint 2, a rotary motor 3, and a micromanipulation module 4. The rotary motor 3 is provided with an external threaded rotation The shaft 5 is provided with a through hole in the middle of the bottom of the rotary joint 2, and the rotary shaft 5 passes through the through hole and extends out of the rotary joint 2. The rest of the bottom of the rotary joint 2 is a sealed structure, and the upper part of the rotary joint 2 is connected to the microfluidic control unit. In the lower part of the culture chip set 1, the rotating motor 3 is located below the rotary joint 2, the rotating motor 3 is fixedly connected to the microfluidic culture chip set 1 through the rotating shaft 5, and the micromanipulation module 4 is connected to the microfluidic culture chip set 1; the present invention The device also i...

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Abstract

The invention discloses a micro-fluidic-technique-based living cell culture device which comprises a micro-fluidic culture chip set, a rotary joint, a rotary motor, a micromanipulation module and a fluidic pump, wherein the rotary joint is in butt joint with the bottom the micro-fluidic culture chip set, the rotary motor is arranged below the rotary joint, a rotary shaft is arranged on the rotary motor, the rotary shaft penetrates through and extends out of the rotary joint, the rotary motor is fixedly connected with the micro-fluidic culture chip set through the rotary shaft, the micromanipulation module is connected with the micro-fluidic culture chip set, and the fluidic pump is communicated with the micro-fluidic culture chip set by virtue of the rotary joint. The device disclosed by the invention can be used for simultaneously carrying out in vitro sequential culture of tens of cells/embryos and micro-intervening operation on the surrounding environment of a target and the living body.

Description

technical field [0001] The invention relates to a living cell culture device based on microfluidic technology. Background technique [0002] Cells are the smallest unit of life activity, and the study of cells in vivo is difficult but of great significance. Live observation of embryos can obtain a lot of dynamic information in the process of life generation and development, which is also of great significance. By 2012, more than 4 million test-tube babies have been born around the world, and the number is increasing at a rate of more than 100,000 per year; there are more than 1 million in vitro fertilization (IVF) cycles in the world every year; in the United States, oocytes The number of cycles of intrapulmonary microinjection (ICSI) accounts for about 57.5% of all IVF cycles. The latest survey shows that infertile couples in my country have also accounted for 15% of the total number of couples of childbearing age. At present, the medical expenses of a conventional IVF cy...

Claims

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Application Information

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IPC IPC(8): C12M3/00
CPCC12M23/02C12M23/42C12M25/04C12Q1/22
Inventor 范晶何农跃吴丹陈慧邬燕琪
Owner SOUTHEAST UNIV
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