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Diagnostic method for assessing state of atopic dermatitis by CTACK/CCL27

A technology for CCL27 and diagnostic methods, applied in the field of molecular biology, can solve the problems of complex evaluation methods and SCORAD is not widely used, and achieve the effect of reducing the burden and increasing the accuracy

Inactive Publication Date: 2014-12-03
SHENZHEN PKU HKUST MEDICAL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, SCORAD is not widely used in clinical practice due to the complexity of the assessment method

Method used

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  • Diagnostic method for assessing state of atopic dermatitis by CTACK/CCL27
  • Diagnostic method for assessing state of atopic dermatitis by CTACK/CCL27

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0013] The present invention is further explained below in conjunction with the examples, but the examples do not limit the present invention in any form.

[0014] 1. Coat the CTACK / CCL27 antibody on the solid support. Incubate for a certain period of time to allow the formation of solid-phase antibodies, and wash to remove unbound antibodies and impurities.

[0015] 2. Add the sample to be tested and incubate to make the antigen in the sample fully react with the antibody on the solid-phase carrier to form a solid-phase antigen-antibody complex. Washing removes other unbound material.

[0016] 3. Add enzyme-labeled anti-CTACK / CCL27 antibody and incubate to form a solid-phase antibody to be tested antigen-enzyme-labeled antibody sandwich complex. Wash to remove unbound enzyme-labeled antibody.

[0017] 4. Add substrate to develop color. The enzyme on the solid phase catalyzes the substrate to produce a colored product, and the amount of antibody in the sample is measured c...

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Abstract

The invention discloses a diagnostic method for assessing the state of atopic dermatitis by CTACK / CCL27. According to the method disclosed by the invention, by using the high correlation between the content of CTACK / CCL27 protein in the blood serum of a patient and an SCORAD scoring method used in general diagnosis, the content of the CTACK / CCL27 protein in the blood serum of the patient suffering from atopic dermatitis is assayed by using Elisa instead of judging the severity of the state of the patient suffering from atopic dermatitis by the SCORAD scoring method in the prior art. By using the method disclosed by the invention, the workload of doctors can be greatly reduced, the influence caused by subjective factors during diagnosis is lowered, the accuracy of diagnosis and dosage is improved, and thus, good news is brought for the patient.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a diagnostic method for evaluating the condition of specific dermatitis by CTACK / CCL27. Background technique [0002] Atopic dermatitis is a chronic skin disease with a high incidence, and the diagnosis of atopic dermatitis is not difficult. To achieve effective treatment of atopic dermatitis, its accurate diagnosis and condition assessment are essential to reach a broad consensus that standard therapy has also been announced. judge. [0003] For the symptoms and severity of atopic dermatitis, although the SCORAD score was introduced to evaluate the skin to indicate symptoms and development, the severity can be divided into mild, moderate and severe based on this method. However, SCORAD is not widely used in clinical practice due to the complexity of the assessment method. [0004] CTACK / CCL27 cDNA encodes a protein precursor of 112 amino acids, of which the first 24 amino aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6881
Inventor 张伟魏准于波窦侠杨旸欧国敏
Owner SHENZHEN PKU HKUST MEDICAL CENT