Function and application of leukocyte immunoglobulin-like receptor subfamily b member 4 in the treatment of atherosclerosis

A technique for the treatment of atherosclerosis, which is applied in the field of gene function and application, can solve the unexplained problems of biological function and possible mechanism of action, and achieve the effect of inhibiting atherosclerosis

Active Publication Date: 2016-09-14
武汉惠康基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the above research basis, we believe that LILRB4 may play an important role in the characteristic pathophysiological process of atherosclerosis, but its biological function and possible role in the characteristic pathophysiological process of atherosclerosis mechanism has not been elucidated

Method used

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  • Function and application of leukocyte immunoglobulin-like receptor subfamily b member 4 in the treatment of atherosclerosis
  • Function and application of leukocyte immunoglobulin-like receptor subfamily b member 4 in the treatment of atherosclerosis
  • Function and application of leukocyte immunoglobulin-like receptor subfamily b member 4 in the treatment of atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Mouse Atherosclerosis Model (AS) Obtained

[0033] 1. Grouping of experimental animals: 8 weeks old, weighing 19-25g, male, ApoE - / - Mice and LILRB4 - / - ApoE - / - Mice were fed high-fat diet (Western Diets, HFD) and low-fat diet (Normal chow, NC) respectively, ApoE - / - HFD group, ApoE - / - NC group, LILRB4 - / - ApoE - / - HFD group, LILRB4 - / - ApoE - / - The NC group consisted of 4 groups with 20 rats in each group.

[0034] 2. Atherosclerosis model induced by high-fat diet:

[0035] Using ApoE - / - Mice and LILRB4 - / - ApoE - / - In mice, AS models were established, and phenotype correlation analysis was performed to clarify the role of LILRB4 gene in atherosclerotic diseases. From the age of 8 weeks, the mice in the HFD group were sacrificed after 28 weeks of high-fat diet, and the samples were collected in the NC group after 28 weeks of low-fat diet.

Embodiment 2

[0036] Example 2 Determination of plaque area in AS model mice

[0037] 1. Final mouse tissue harvesting

[0038] Mice were fed with high-fat or low-fat diet until 28 weeks, weighed, anesthetized with 3% pentobarbital sodium, 90 mg / kg, fixed on the sampling board with a needle, moistened the skin of the chest and abdomen of the mouse with gauze, and used Ophthalmic scissors cut open the chest cavity, expose the heart, cut open the right atrial appendage, insert the needle of the infusion set into the left ventricle, inject 10-15mL PBS buffer slowly with a 50mL syringe, wait until the right atrial appendage effluent is clear, replace it with 4% Polymer Formaldehyde continued to inject 10-15mL. After the perfusion, the thoracoabdominal viscera were removed and only the heart was kept. Put the mouse under a microscope, separate the fascia and fatty tissue around the aortic arch, cut the heart at the beginning of the ascending aorta, cut at the middle of the thoracic aorta, and ...

Embodiment 3

[0051] Example 3 Determination of Plaque Stability in AS Model Mice

[0052] 1. Area Size Determination of the Center of Aortic Sinus Necrosis

[0053] For hematoxylin and eosin staining (HE staining), the method is the same as in Example 2.4, and a tissue containing cholesterol crystals and no nucleus fiber structure is selected to take pictures under a microscope.

[0054] Determination of the area of ​​the necrosis center: use Image-Pro Plus 6.0 image analysis software to circle the area of ​​the necrosis center.

[0055] 2. Determination of collagen content in aortic sinus:

[0056] Sirius red (PSR) staining, the main steps are: take aortic sinus paraffin white slices and bake at 55°C for 30 minutes → xylene for 2 minutes, 3 times → 100% alcohol for 1 minute → 95% alcohol for 1 minute → 70% alcohol for 1 minute → rinse with running water for 10 minutes → Double distilled water for 1min→mass fraction 0.2% phosphomolybdic acid for 2min→0.1% Sirius red picric acid solution ...

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Abstract

The invention discloses the function and application of a leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) in the treatment of atherosclerosis, and belongs to the field of gene function and application. The present invention uses ApoE ‑ / ‑ Mice and LILRB4 ‑ / ‑ ApoE ‑ / ‑ Mice were used as experimental subjects to obtain an atherosclerosis model induced by a high-fat diet. ‑ / ‑ Compared with mice, LILRB4 gene deficiency significantly increased the plaque area of ​​the aorta, decreased the stability of the plaque in the aortic sinus, and significantly worsened the inflammatory response. This indicates that the function of LILRB4 in atherosclerosis is mainly reflected in that LILRB4 has the effect of inhibiting the formation of aortic plaques, especially LILRB4 can inhibit the effect of atherosclerosis. Aiming at the above-mentioned functions of LILRB4, LILRB4 can be used to prepare a drug for preventing, relieving and / or treating atherosclerosis.

Description

technical field [0001] The invention belongs to the field of gene function and application, and specifically relates to the function and application of a leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) in the treatment of atherosclerosis, specifically LILRB4 in the preparation of prevention, relief and / or in the application of drugs for the treatment of atherosclerosis. Background technique [0002] Cardiovascular and cerebrovascular diseases are the main cause of death in many developed countries, and the morbidity and mortality in my country are also increasing year by year. The basis of cardiovascular and cerebrovascular diseases is atherosclerosis (Atheosclersis, AS). Atherosclerosis can thicken and harden the arterial wall and narrow the lumen, leading to many cardiovascular and cerebrovascular events. Acute stenosis and occlusion of coronary arteries caused by rupture of atherosclerotic unstable plaques, platelet aggregation, and thrombus formati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P9/10
Inventor 李红良郭森蒋丁胜程文林
Owner 武汉惠康基因科技有限公司
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