Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Stabilising and analysing fatty acids in a biological sample stored on solid media

一种固体介质、脂肪酸的技术，应用在产生脂肪、测试用样品的制备、分析材料等方向，能够解决血斑方法不实用等问题

Inactive Publication Date: 2014-12-31
ADELAIDE RES & INNOVATION PTY LTD
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Oxidation of long-chain polyunsaturated fatty acids therefore renders this blood spot method impractical since it would be necessary to analyze all samples at a fixed time after collection if results were to be compared

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Stabilising and analysing fatty acids in a biological sample stored on solid media
  • Stabilising and analysing fatty acids in a biological sample stored on solid media
  • Stabilising and analysing fatty acids in a biological sample stored on solid media

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0127] Example 1 - Stabilization of blood spots on Whatman silica gel chromatography paper using a combination of BHT and ascorbic acid / EDTA

[0128] Materials and methods

[0129] blood collection paper

[0130] Two types of paper were used for blood spot collection: Fruka blood collection kit paper (Sigma-Aldrich, Buchs, Switzerland) and Waterman silica gel chromatography paper (46 × 57 cm, Waterman, Buckingham, UK). Two commercial phenolic antioxidants, butylated hydroxytoluene and tert-butylhydroquinone (TBHQ), were purchased from Sigma-Aldrich (St Louis, MO). Iron chelator, L-ascorbic acid and EDTA were purchased from Chem-supply Company (Gillman, South Australia).

[0131] Antioxidant / Chelating Agent (Protectant) Solution

[0132] Fourteen protectant formulations were prepared to stabilize fatty acids in blood spots on two types of collection paper (Table 1). In order to determine the optimal concentration of antioxidants to control lipid oxidation in blood spots,...

example 2

[0161] Example 2 - Stabilization of plasma plaques on Whatman silica gel chromatography paper using a combination of BHT and EDTA

[0162] Blood was collected from subjects who regularly consumed one fish oil capsule per day. The blood samples were centrifuged at 3000 rpm for 10 minutes at 4°C to separate plasma and erythrocytes. A drop of plasma (approximately 50 μl) was directly transesterified and the resulting FAMEs analyzed by GC to provide a baseline value for the fatty acid composition in plasma total lipids. The remaining plasma was split in two, the first stored at -20°C and tested for fatty acid composition by direct transesterification at 1, 2 and 4 weeks. The second aliquot was dropped onto Waterman silica gel chromatography paper (46 x 57 cm, Waterman, Buckinghamshire, UK) soaked with 2 mg / ml BHT and 5 mg / ml EDTA (approximately 50 μl per paper), And dry in air for 5-6 hours. Plasma spots were then stored in cellophane bags at room temperature (19°C-23°C) in the...

example 3

[0167] Example 3 - Stabilization of milk spots on Whatman silica gel chromatography paper using a combination of BHT and EDTA

[0168]Milk was obtained from subjects who had been lactating for 5 months and were not receiving fish oil supplementation. A drop of milk (approximately 50 μl) was directly transesterified and the resulting FAMEs analyzed by GC to provide a baseline value for the fatty acid composition of the milk's total lipid. Drop the rest of the milk onto Waterman silica gel chromatography paper (46×57cm, Waterman Company, Buckinghamshire, UK) soaked with 2mg / ml BHT and 5mg / ml EDTA (about 50 μl per piece of paper), and air Dry in medium for 5-6 hours. Once the milk spots are dry, divide them into 4 groups. The first group was immediately transesterified following the same transesterification procedure as for fresh milk. The results obtained from these samples were compared with those obtained by direct measurement of the fatty acid composition of fresh milk to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for stabilising fatty acids present in a sample such as bodily fluids (e.g. blood, saliva, breast milk, urine, semen, blood plasma and serum), using a solid medium (such as paper, a glass-based matrix, a paper-based matrix, a cellulose-based matrix, hydrophilic polymers, polytetrafluoroethylene, fibreglass and porous ceramics) comprising chelating agents (e.g. ethylenediamine-tetraacetic acid, ascorbic acid, citric acid, or salts thereof), antioxidants (e.g. butylated hydroxytoluene, butylated hydroxyanisole or t-butylhydroquinone) and less than than about 2 mug / cm2 of contaminants which is capable of stabilising fatty acids applied thereto, and a method for preparing such media. The invention further relates to a method for determining the fatty acid composition of a sample stored on such a medium.

Description

technical field [0001] The present invention relates to a method for stabilizing fatty acids present in a sample, such as a body fluid. The invention further relates to a solid medium capable of stabilizing fatty acids applied thereto, and a method for its preparation. The invention further relates to a method for determining the fatty acid composition of a sample. Background of the invention [0002] High intakes of omega-3 fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have been associated with reduced rates of cardiovascular disease, neurodegenerative disease, diabetes, and arthritis in adults and improved mental development in preterm infants. Therefore, health authorities all over the world now recommend increasing the intake of omega-3 fatty acids, for example by consuming a meal including fish 2 to 3 times a week. [0003] Dietary omega-3 fatty acid recommendations for cardioprotection include increasing fish intake to two fatty...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/50G01N33/48G01N33/92A61B5/15C11B5/00G01N1/36
CPCA61B5/15C11B5/00A61B5/150305A61B5/150358A61B5/150755G01N2030/8813C11B5/0007A61B5/15003F04C2270/0421G01N33/487G01N33/49G01N33/493G01N1/36G01N30/50G01N33/48G01N33/92C09K15/22
Inventor 罗伯特·吉布森刘·戈
Owner ADELAIDE RES & INNOVATION PTY LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com