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A kind of preparation method of effective part of Dendrobium officinale

A technology of effective parts, Dendrobium officinale, applied in the field of medicine, can solve the problems of inability to extract, waste of resources, poor curative effect, etc., and achieve the effects of improved activity, high extraction rate, and improved utilization efficiency

Active Publication Date: 2017-04-26
NINGBO FENGKANG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional Dendrobium officinale-related products, including various small compound health products, tea maple buckets, and polysaccharides, are all water-extracted or water-extracted concentrated extracts. This traditional water-extracted process obviously cannot exert anti-tumor and anti-inflammatory effects. Bibenzyl compounds with important pharmacological activities such as oxidation and other fat-soluble components are well extracted, resulting in extensive product technology, poor curative effect, and serious waste of resources

Method used

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  • A kind of preparation method of effective part of Dendrobium officinale

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[0021] In an embodiment of the present invention, a method for preparing effective parts of Dendrobium officinale comprises extraction of fat-soluble total bibenzyl and water-soluble total polysaccharides. The extraction step of the fat-soluble total bibenzyl is as follows: adding 8-12 times the amount of 65%-95% high-concentration ethanol aqueous solution to Dendrobium officinale according to the solid-liquid ratio, heating to 70-100°C, and refluxing extraction for 2-3 times, 2-3 hours each time; combine the extracts, concentrate under reduced pressure to obtain a fat-soluble extract; dissolve the extract with an appropriate amount of ethanol, then add it to a macroporous resin column, and take the 25%-75% ethanol aqueous solution eluting part, Concentrate to obtain the effective parts of the total bibenzyl; the extraction step of the water-soluble total polysaccharide is: use the residue left in the extraction step of the fat-soluble total bibenzyl as raw material, add 8-12 t...

Embodiment 1

[0065] Preparation of effective fraction of total bibenzyl

[0066] Dry 1 kg of Dendrobium candidum, add 12L of 80% ethanol, soak overnight, heat and reflux at 92°C for extraction twice, each time for 3 hours, combine the extracts, recover the solvent until there is no alcohol smell to obtain 98g of extract and dry it, which is the fat-soluble extract; Dissolve with 30ml of 95% ethanol, put it on the D101 type macroporous resin, take 40%-60% of the eluted parts, concentrate and dry under reduced pressure to obtain 46g of total bibenzyl effective parts, and measure the effective parts by ultraviolet spectrophotometry The bibenzyl content in is 60.5%.

Embodiment 2

[0068] Preparation of total effective fractions of Dendrobium officinale

[0069] The total polysaccharides were prepared by extracting the traditional Chinese medicine residue of Example 1 by water extraction and alcohol precipitation, that is, heating 12L of water to 100°C for extraction twice, each time for 2h; Precipitate polysaccharides, filter, and dry to obtain 42 g of total polysaccharides. Take 23 g of the above-mentioned total bibenzyls and combine them with 21 g of total polysaccharides to obtain 44 g of total active parts, which is equivalent to 500 g of the original medicinal material.

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Abstract

The invention discloses a preparation method of an effective part of dendrobium officinale. The method comprises steps as follows: firstly, extracting fat-soluble extracts represented by bibenzyl from the dendrobium officinale with a high-concentration alcohol-water system, refining the extracts with a macroporous resin method to obtain a total bibenzyl effective part with the bibenzyl content higher than 50%, and preparing dregs of the extracts into dendrobium officinale total polysaccharides with water extraction and alcohol precipitation methods; drying and evenly mixing the total bibenzyl effective part and the dendrobium officinale total polysaccharides obtained in two steps to obtain a dendrobium officinale total effective part extract. According to the method, the defect of the extremely low extraction rate of dendrobium officinale fat-soluble active ingredients represented by the bibenzyl with traditional methods such as a water decoction method, a tea making method and the like is overcome, the utilization efficiency of the effective part of the dendrobium officinale is improved substantially, pharmacological anti-oxidation animal experiments and anti-tumor cytotoxicity experiments prove that the activity of the effective part is significantly higher than that of traditional aqueous extracts, and the effective part has the characteristics of high extraction rate and high activity.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for preparing effective parts of dendrobium officinale. Background technique [0002] Dendrobium candidum is a perennial herbaceous plant of the family Dendrobium. Its main medicinal parts are fresh or dried stems. It is mainly distributed in Anhui, Zhejiang, Guangxi, Hunan, Yunnan, Guizhou and other places. It is a traditional precious Chinese medicinal material. Dendrobium officinale has the effect of benefiting the stomach and promoting body fluid, nourishing yin and clearing away heat. It is used for yin injury and fluid deficiency, dry mouth and polydipsia, insufficient stomach yin, retching due to lack of food, persistent deficiency heat after illness, hyperactivity of fire due to yin deficiency, fatigue and heat due to bone steaming, Obscurity of eyesight, muscle and bone flaccidity and weakness embolisms. "Shen Nong's Materia Medica" listed it as the top grade,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8984C08B37/00A61P39/06A61P35/00
CPCA61K36/8984A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55C08B37/0003
Inventor 陈贤良
Owner NINGBO FENGKANG BIOLOGICAL TECH
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