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A strain of lignin-tolerant Saccharomyces cerevisiae and its application in bioethanol production

A technology of Saccharomyces cerevisiae and Saccharomyces cerevisiae, which is applied in the field of lignin-tolerant Saccharomyces cerevisiae and its application in bioethanol production, which can solve the problems of low industrial application, low removal rate of toxic components, and high processing cost. problems, to achieve the effects of promoting industrial development, improving fermentation efficiency, and strengthening ethanol fermentation capacity

Inactive Publication Date: 2017-10-10
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have problems such as high processing cost, low removal rate of toxic components, and large loss of sugar, so their industrial application is not high.

Method used

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  • A strain of lignin-tolerant Saccharomyces cerevisiae and its application in bioethanol production
  • A strain of lignin-tolerant Saccharomyces cerevisiae and its application in bioethanol production
  • A strain of lignin-tolerant Saccharomyces cerevisiae and its application in bioethanol production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 This example illustrates the screening method for Saccharomyces cerevisiae LTY-1 of the present invention.

[0031] The culture medium adopted in the screening of the present invention is:

[0032] (1) Liquid YPD medium: yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, natural pH.

[0033] (2) Solid YPD medium: 20 g / L of agar was added to the liquid YPD medium.

[0034] (3) Solid regeneration medium: 20 g / L of agar and 170 g / L of sucrose were added to the liquid YPD medium.

[0035] (4) Seed medium: yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, natural pH.

[0036] (5) Fermentation medium: yeast extract powder 61g / L, peptone 10g / L, glucose 350g / L, natural pH.

[0037] Saccharomyces cerevisiae (Saccharomyces cerevisiae) CGMCC2.1429 (China General Microorganism Collection Center) was used as the original yeast species. After the strains were purchased, they were transferred from the ampoule tube to the slant-plane YPD medium, cultured in...

Embodiment 2

[0061] Example 2 This example illustrates the ethanol fermentation ability of Saccharomyces cerevisiae LTY-1 under different conditions of initial sugar concentration, fermentation time, fermentation temperature and shaker speed.

[0062] 1) Yeast strain

[0063] (1) Original yeast strain (Saccharomyces cerevisiae) CGMCC2.1429: provided by China Microorganism Culture Collection and Management Center.

[0064] (2) Lignin-tolerant Saccharomyces cerevisiae LTY-1: cultivated by the present inventors.

[0065] 2) Medium

[0066] (1) Seed medium: yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, natural pH.

[0067] 3) Determination of the growth curve of Saccharomyces cerevisiae LTY-1

[0068] 50 mL of seed medium was divided into 250 mL Erlenmeyer flasks, and Saccharomyces cerevisiae LTY-1 was transferred from the slant YPD medium to the seed medium respectively, and cultured at 30°C and 180 rpm for 48 hours. During the cultivation process, take out one Erlenmeyer flas...

Embodiment 3

[0079] Example 3 This example illustrates the ethanol fermentation ability of Saccharomyces cerevisiae LTY-I in a lignin-containing fermentation medium.

[0080] 1) Yeast strain

[0081] (1) Original yeast strain (Saccharomyces cerevisiae) CGMCC2.1429: provided by China Microorganism Culture Collection and Management Center.

[0082] (2) Lignin-tolerant Saccharomyces cerevisiae LTY-1: cultivated by the present inventors.

[0083] 2) Medium

[0084] (1) Seed medium: yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, natural pH.

[0085] (2) Lignin fermentation medium: yeast extract powder 61g / L, peptone 10g / L, glucose 242g / L, syringol 1.44g / L, p-hydroxybenzaldehyde 1.44g / L, vanillin 1.44g / L, pH5.5.

[0086] 3) Ethanol fermentation

[0087] Seed culture: Saccharomyces cerevisiae LTY-1 was cultured to the mid-logarithmic phase under the conditions of seed medium, 30° C., and 180 rpm, and used as seed liquid.

[0088] Ethanol fermentation: the seed liquid was transfer...

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Abstract

The invention discloses a strain of Saccharomyces cerevisiae LTY‑1 (Saccharomyces cerevisiae LTY‑1) capable of enduring lignin toxicity, which has been preserved in the China Center for Type Culture Collection (CCTCC for short) on May 6, 2014, and its preservation number is It is CCTCC NO: M 2014184. When using the fermentation medium of 242g / L glucose, 10g / L peptone, 61g / L yeast extract powder, and pH 5.5, and fermented for 36 hours at 30°C and 150rpm, the sugar conversion rate of Saccharomyces cerevisiae LTY‑1 was 84.4%. When the fermentation medium contained lignin components such as syringyl alcohol, p-hydroxybenzaldehyde and vanillin, and the concentration of each component was 1.44g / L, the sugar conversion rate of Saccharomyces cerevisiae LTY‑1 was only reduced by 7.0%. The degree of reduction was far less than 18.5% of the original yeast. The core technology and materials are provided for improving the ethanol fermentation efficiency of lignocellulose hydrolyzate. The technology of the invention can be applied to bioethanol production with lignocellulose as a raw material, and has great significance for promoting the industrialization development of bioethanol production, environmental protection, and food security.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and specifically relates to a strain of lignin-tolerant Saccharomyces cerevisiae and its application in bioethanol production. Background technique [0002] Liu Gang , Shen Lei. Quantitative evaluation of biomass energy in China and its geographical distribution [J]. Journal of Natural Resources. 2007,41(3):9~19). The production and utilization of bioethanol from lignocellulose is considered to be one of the effective ways to solve energy, environment and food problems. All countries in the world are focusing on the development of common key technologies and special equipment in the production process of lignocellulosic bioethanol to actively promote the development of the bioenergy industry. [0003] The production process of lignocellulosic bioethanol generally includes the main steps of raw material pretreatment, enzymatic hydrolysis, ethanol fermentation and ethanol refining...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12P7/06C12P7/10C12R1/865
CPCC12P7/06C12P7/10C12N1/185C12R2001/865Y02E50/10
Inventor 缪冶炼尤业兵季萌陈君李文莉许琳
Owner NANJING TECH UNIV
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