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Lignin-tolerant saccharomyces cerevisiae and application of lignin-tolerant saccharomyces cerevisiae in production of bioethanol

A technology of Saccharomyces cerevisiae and Saccharomyces cerevisiae, which is applied to lignin-tolerant Saccharomyces cerevisiae and its application field in bioethanol production, can solve the problem of low degree of industrial application, low removal rate of toxic components and high processing cost problems, to achieve the effect of promoting industrialization development, improving fermentation efficiency, and strengthening ethanol fermentation capacity

Inactive Publication Date: 2015-01-07
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have problems such as high processing cost, low removal rate of toxic components, and large loss of sugar, so their industrial application is not high.

Method used

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  • Lignin-tolerant saccharomyces cerevisiae and application of lignin-tolerant saccharomyces cerevisiae in production of bioethanol
  • Lignin-tolerant saccharomyces cerevisiae and application of lignin-tolerant saccharomyces cerevisiae in production of bioethanol
  • Lignin-tolerant saccharomyces cerevisiae and application of lignin-tolerant saccharomyces cerevisiae in production of bioethanol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 This example illustrates the screening method for Saccharomyces cerevisiae LTY-1 of the present invention.

[0031] The culture medium adopted in the screening of the present invention is:

[0032] (1) Liquid YPD medium: yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, natural pH.

[0033] (2) Solid YPD medium: 20 g / L of agar was added to the liquid YPD medium.

[0034] (3) Solid regeneration medium: 20 g / L of agar and 170 g / L of sucrose were added to the liquid YPD medium.

[0035] (4) Seed medium: yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, natural pH.

[0036] (5) Fermentation medium: yeast extract powder 61g / L, peptone 10g / L, glucose 350g / L, natural pH.

[0037] Saccharomyces cerevisiae (Saccharomyces cerevisiae) CGMCC2.1429 (China General Microorganism Collection Center) was used as the original yeast species. After the strains were purchased, they were transferred from the ampoule tube to the slant-plane YPD medium, cultured in...

Embodiment 2

[0061] Example 2 This example illustrates the ethanol fermentation ability of Saccharomyces cerevisiae LTY-1 under different conditions of initial sugar concentration, fermentation time, fermentation temperature and shaker speed.

[0062] 1) Yeast strain

[0063] (1) Original yeast strain (Saccharomyces cerevisiae) CGMCC2.1429: provided by China Microorganism Culture Collection and Management Center.

[0064] (2) Lignin-tolerant Saccharomyces cerevisiae LTY-1: cultivated by the present inventors.

[0065] 2) Medium

[0066] (1) Seed medium: yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, natural pH.

[0067] 3) Determination of the growth curve of Saccharomyces cerevisiae LTY-1

[0068] A 250mL conical flask was used to aliquot 50mL of seed medium, and Saccharomyces cerevisiae LTY-1 was transferred from the slant YPD medium to the seed medium respectively, and cultured at 30°C and 180rpm for 48h. During the cultivation process, take out one Erlenmeyer flask every...

Embodiment 3

[0079] Example 3 This example illustrates the ethanol fermentation ability of Saccharomyces cerevisiae LTY-I in a lignin-containing fermentation medium.

[0080] 1) Yeast strain

[0081] (1) Original yeast strain (Saccharomyces cerevisiae) CGMCC2.1429: provided by China Microorganism Culture Collection and Management Center.

[0082] (2) Lignin-tolerant Saccharomyces cerevisiae LTY-1: cultivated by the present inventors.

[0083] 2) Medium

[0084] (1) Seed medium: yeast extract powder 10g / L, peptone 20g / L, glucose 20g / L, natural pH.

[0085] (2) Lignin fermentation medium: yeast extract powder 61g / L, peptone 10g / L, glucose 242g / L, syringol 1.44g / L, p-hydroxybenzaldehyde 1.44g / L, vanillin 1.44g / L, pH5.5.

[0086] 3) Ethanol fermentation

[0087] Seed culture: Saccharomyces cerevisiae LTY-1 was cultured to the mid-logarithmic phase under the conditions of seed medium, 30° C., and 180 rpm, and used as seed liquid.

[0088] Ethanol fermentation: the seed liquid was transfer...

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Abstract

The invention discloses a saccharomyces cerevisiae LTY-1 capable of tolerating the toxicity of lignin. The saccharomyces cerevisiae LTY-1 is preserved in China Center for Type Culture Collection (referred to as CCTCC) on May 6, 2014 and the preservation number is CCTCC NO:M2014184. When a fermentation culture medium which contains 242g / L of glucose, 10g / L of peptone, 61g / L of yeast extract powder and of which the pH value is 5.5 is adopted, the saccharomyces cerevisiae LTY-1 is fermented for 36 hours under the conditions of 30 DEG C and 150rpm and the glucose conversion rate of the saccharomyces cerevisiae LTY-1 is 84.4%. When the fermentation culture medium contains lignin components, such as syringol, p-hydroxybenzaldehyde and vanillin and the concentration of each component is 1.44g / L, the glucose conversion rate of the saccharomyces cerevisiae LTY-1 is only reduced by 7.0% which is far less than 18.5% of the original yeast. The saccharomyces cerevisiae LTY-1 provides the core technology and materials for improving the fermentation efficiency of ethanol in lignocellulosic hydrolyzate. The saccharomyces cerevisiae LTY-1 can be applied in production of bioethanol from lignocelluloses as a feedstock and is of great significance for promoting industrial development of bioethanol production, environmental friendliness and grain safety guarantee.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and specifically relates to a strain of lignin-tolerant Saccharomyces cerevisiae and its application in bioethanol production. Background technique [0002] Liu Gang , Shen Lei. Quantitative evaluation of biomass energy in China and its geographical distribution [J]. Journal of Natural Resources. 2007,41(3):9~19). The production and utilization of bioethanol from lignocellulose is considered to be one of the effective ways to solve energy, environment and food problems. All countries in the world are focusing on the development of common key technologies and special equipment in the production process of lignocellulosic bioethanol to actively promote the development of the bioenergy industry. [0003] The production process of lignocellulosic bioethanol generally includes the main steps of raw material pretreatment, enzymatic hydrolysis, ethanol fermentation and ethanol refining...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12P7/06C12P7/10C12R1/865
CPCC12P7/06C12P7/10C12R2001/865C12N1/185Y02E50/10
Inventor 缪冶炼尤业兵季萌陈君李文莉许琳
Owner NANJING UNIV OF TECH
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