Method for rapidly detecting pest resistance of barnyard grass

Abstract

The invention discloses a method for rapidly detecting the pest resistance of barnyard grass. The method comprises the following steps: (1) collecting barnyard grass seeds sensitive to a weed killer, wherein the barnyard grass seeds sensitive to the weed killer as materials; (2) culturing the barnyard grass, namely breaking the dormancy of the barnyard grass seeds collected in the step 1, firstly soaking the seeds by using potassium nitrate for germination, putting germinated barnyard grass into a nutrient solution and culturing until two leaves grow for spare use; and (3) treating the barnyard grass, namely sticking the barnyard grass with the two leaves in the step (2) on a plastic board, then putting the barnyard grass into weed-killer liquors with different gradient concentrations, soaking for 30 minutes, then taking out, naturally drying, putting into the nutrient solution for culturing continuously for 15 days, evaluating the change of the color of the leaves, the growth of new leaves and the development of the root system of the barnyard grass, and surveying the death rate of the barnyard grass, so as to obtain the screening concentration of the weed killer; and therefore, whether the barnyard grass generates the resistance to the weed killer can be rapidly detected. The method disclosed by the invention can be used for detecting a large batch of barnyard grass samples one time, and has the advantages of short time, accuracy and reliability.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for quickly detecting whether barnyardgrass is resistant to herbicides. Background technique [0002] barnyardgrass ( Echinochloa crusgalli ) is one of the top ten malignant weeds in the world, and it is also one of the most serious weeds on the main crops in our country. The harm to rice production is particularly serious, not only the area of ​​damage is large, but also the most significant reduction in rice production. [0003] At present, chemical control is still the most important way to control barnyardgrass. Because barnyardgrass is widely distributed in farmland around the world, it is very harmful. Therefore, the development of herbicides mostly takes barnyardgrass as the main control target, and various types of barnyard grasses with different mechanisms of action have been developed one after another. [0004] However, barnyardgrass has developed...

AI Technical Summary

Problems solved by technology

However, some methods use expensive detection instruments, such as chloroplast resistance assay and fluorescent signal detection method

There are also some methods that involve expensive test reagents that require professional technicians to complete, such as the target resistance molecular detection method