Method for rapidly detecting pest resistance of barnyard grass
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A drug resistance and barnyardgrass technology, which is applied in the field of rapid detection of barnyardgrass resistance to herbicides, can solve the problems of expensive test reagents and expensive detection instruments
Active Publication Date: 2015-01-07
HUNAN PLANT PROTECTION INST
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Problems solved by technology
However, some methods use expensive detection instruments, such as chloroplast resistance assay and fluorescent signal detection method
There are also ...
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Embodiment 1
[0023] Rapid detection method of barnyardgrass resistance to Quinclorac
[0024] First break the dormancy of the collected barnyard grass seeds, soak them in 0.25% potassium nitrate at room temperature for 1 day, filter out the excess water, and then place them in an incubator. After they germinate, put them in a concentration of 0.526ml / ml Feiwanjia Continue to cultivate in the nutrient solution. When the cultivation reaches the 1-leaf stage, stick 10 barnyardgrass seedlings on a 15cm long and 7cm wide plastic plate, and then place the plastic plate obliquely in a cup containing Feiwanjia nutrient solution Continue to grow, keep the plant roots immersed in water during cultivation, and wait until the barnyardgrass has grown to 2 leaves, and then set aside.
[0025] Screening of screening concentration:
[0026] Taking the field dose of Quinclorac as a reference, first set up 6 treatments with a 10-fold gradient of 0, 0.05, 0.5, 5, 50, 500, 5000g a.i. / hm 2 , Each treatment was repe...
Embodiment 2
[0032] Rapid detection method of barnyardgrass resistance to penoxsulam
[0033] First break the dormancy of the collected barnyard grass seeds, soak them in 0.25% potassium nitrate at room temperature for 1 day, filter out the excess water, and then place them in an incubator. After they germinate, put them in a concentration of 0.526ml / ml Feiwanjia Continue to cultivate in the nutrient solution. When the cultivation reaches the 1-leaf stage, stick 10 barnyardgrass seedlings on a 15cm long and 7cm wide plastic plate, and then place the plastic plate obliquely in a cup containing Feiwanjia nutrient solution Continue to grow, keep the plant roots immersed in water during cultivation, and wait until the barnyardgrass has grown to 2 leaves, and then set aside.
[0034] Screening of screening concentration:
[0035] Taking the field dose of penoxsulam as a reference, first set up 6 treatments with a 10-fold gradient of 0, 0.0025, 0.025, 0.25, 2.5, 25, 250 g a.i. / hm 2 , Each treatment ...
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Abstract
The invention discloses a method for rapidly detecting the pest resistance of barnyard grass. The method comprises the following steps: (1) collecting barnyard grass seeds sensitive to a weed killer, wherein the barnyard grass seeds sensitive to the weed killer as materials; (2) culturing the barnyard grass, namely breaking the dormancy of the barnyard grass seeds collected in the step 1, firstly soaking the seeds by using potassium nitrate for germination, putting germinated barnyard grass into a nutrient solution and culturing until two leaves grow for spare use; and (3) treating the barnyard grass, namely sticking the barnyard grass with the two leaves in the step (2) on a plastic board, then putting the barnyard grass into weed-killer liquors with different gradient concentrations, soaking for 30 minutes, then taking out, naturally drying, putting into the nutrient solution for culturing continuously for 15 days, evaluating the change of the color of the leaves, the growth of new leaves and the development of the root system of the barnyard grass, and surveying the death rate of the barnyard grass, so as to obtain the screening concentration of the weed killer; and therefore, whether the barnyard grass generates the resistance to the weed killer can be rapidly detected. The method disclosed by the invention can be used for detecting a large batch of barnyard grass samples one time, and has the advantages of short time, accuracy and reliability.
Description
technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for quickly detecting whether barnyardgrass is resistant to herbicides. Background technique [0002] barnyardgrass ( Echinochloa crusgalli ) is one of the top ten malignant weeds in the world, and it is also one of the most serious weeds on the main crops in our country. The harm to rice production is particularly serious, not only the area of damage is large, but also the most significant reduction in rice production. [0003] At present, chemical control is still the most important way to control barnyardgrass. Because barnyardgrass is widely distributed in farmland around the world, it is very harmful. Therefore, the development of herbicides mostly takes barnyardgrass as the main control target, and various types of barnyard grasses with different mechanisms of action have been developed one after another. [0004] However, barnyardgrass has developed...
Claims
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Application Information
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