Compound microbial agent for urban sewage treatment, preparation method and application thereof
A technology for compounding microbial inoculants and urban sewage, which is applied in the field of sewage treatment and sewage treatment using microbial methods, can solve the problems of difficult to meet discharge standards, difficult targeted treatment, poor biodegradability, etc., and achieve good degradation effect, The effect of improving the treatment effect and improving the biodegradability
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Embodiment 1
[0027] This example is the preparation example of composite microbial bacterial agent.
[0028] Composition and ratio of compound microbial agent: Bacillus subtilis (1~9)×10 10 Individual / g, Bacillus licheniformis (1~9)×10 10 Individual / g, Lactobacillus plantarum (1~9)×10 8 Individual / g, Hansenula (1~9)×10 9 Individual / g, Saccharomyces cerevisiae (1~9)×10 9 pcs / g.
[0029] The above five strains can be obtained from the Guangdong Microbial Culture Collection Center, and the numbers are: Saccharomyces cerevisiae: ATCC26785, Hansenula: AS2.302, Bacillus subtilis: ATCC6633, Bacillus licheniformis: ACCC10236, Lactobacillus plantarum: ATCC8014 .
[0030] Preparation of compound microbial bacterial agent:
[0031] 1. Separate expansion culture of each strain:
[0032] 1) Primary seed cultivation:
[0033] Bacillus subtilis and Bacillus licheniformis were inoculated on nutrient agar slant medium, Lactobacillus plantarum was inoculated on MRS slant medium, and Saccharomyces ce...
Embodiment 2
[0040] This example is to test the effect of compound microbial agent on improving sewage biodegradability and reducing COD cr An experimental example of the value aspect effect.
[0041] (1) The effect of improving the biodegradability of sewage.
[0042] m (BOD 5 ) / m(COD cr ) reflects the biochemical properties of sewage, less than 0.3 indicates that biochemical treatment is not easy, and greater than 0.3 indicates that biochemical treatment is possible. Under the condition of a certain amount of dissolved oxygen, the bacterial agent can decompose macromolecules and refractory substances into biochemical small molecular substances, and improve the biochemical properties of sewage. Take two parts of urban sewage with poor biochemical properties, use a 20L bucket as the test container with stirring, adjust the pH of the sewage to 6.5-7.0, and the water temperature to 25-30°C. Add 2 g of bacterial agents to the test group, but not to the control group. Stop stirring after ...
Embodiment 3
[0050] This example is an application example of using the composite microbial agent of the present invention to treat urban sewage.
[0051] In this example, the composite microbial agent prepared in Example 1 is used to treat urban sewage with poor biochemical properties, using integrated sewage treatment equipment with a volume of 1m 3 , the process is self-flowing aerobic-anaerobic alternation, and the influent water quality is COD cr : 314mg / L, BOD 5 : 64mg / L, before using this bacterial agent, the effluent water quality is COD cr :131mg / L, BOD 5 : 27mg / L. Add 20g of compound microbial agent to the water inlet after activation, and continue to add it for 3-7 days. Stop adding when the filler in the equipment is normally filmed. After treatment, the effluent quality is COD cr :68mg / L, BOD 5 :15.6mg / L, COD cr The removal rate reached 78.3%.
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