Polypeptide molecule and application thereof
A molecular and polypeptide technology, applied in the field of polypeptide molecules and its application, can solve the problems of a large number of antibiotic treatment, high infection rate, removal of implants, etc., and achieve the effect of preventing bacterial infection, obvious application potential, and strong stability.
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[0024] Example 1:
[0025] A polypeptide molecule whose amino acid sequence is shown in SEQ ID No.1;
[0026] SEQ ID No. 1: KKPVPIIYCN KKRGKCQRM.
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[0027] Example 2:
[0028] Preparation of polypeptide molecules:
[0029] Select amino acid-king resin as carrier (resin), fully swell the resin with dichloromethane, wash with dimethylformamide several times, use DBLK of appropriate concentration to remove the Fmoc-protecting group, and then use dimethylformamide to remove the Fmoc-protecting group. The amide was washed several times, DBLK was washed away, and the appropriate condensing agent benzotriazole-N,N,N',N'-tetramethylurea hexafluorophosphate and activator methylmorpholine and C-terminal Two Fmoc-protected amino acids (Fmoc-Pro-OH) were coupled, detected by ninhydrin detection method to ensure that the connection was relatively complete, washed several times with dimethylformamide, washed away various residual residues, activated Coupling agent and condensing agent, according to the amino acid sequence coupling, after connecting all amino acids, remove the last Fmoc-protecting group, cleave with cutting solution, re...
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[0030] Example 3:
[0031] Antibacterial ability test:
[0032] 1. Experimental method
[0033] Add 1 mL concentration of 1×10 to a sterilized test tube 6 1 mg of the polypeptide molecule provided by the present invention was added to the bacterial liquid of each / mL, and after culturing at 37° C. for 24 hours, the culture medium was collected and diluted by doubling, and the dilution factor was 10 times and the number of viable bacteria was detected by coating culture method.
[0034] 2. Experimental results:
[0035] Table 1 Bactericidal rate (%) of the polypeptide molecules of the present invention
[0036] bacteria
[0037] As can be seen from Table 1, the polypeptide molecules provided by the present invention are effective against Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231), Bacillus subtilis black var. (ATCC 9372) ) have strong bactericidal properties, and the bactericidal rate is over 99%.
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