Press-fit device for multi-layer cell culture plate

Abstract

The invention provides a press-fitting structure for multi-layer tissue culture plates. By the press-fitting structure, multiple tissue culture plates which are stacked can be pressed and fixed onto a shaking table, leakproofness and air flowability of plate covers and culture plate bodies of shallow-hole tissue culture plates are ensured, and meanwhile vertical space of the shaking table is utilized effectively. The press-fitting structure comprises a baseplate, a plurality of tissue culture plates are stacked and juxtaposed on the baseplate, a pressing mechanism is arranged on the baseplate and comprises cross shafts, supports, fixing shafts, shaft sleeves and fixing mechanisms, each cross shaft is arranged over the corresponding tissue culture plates in parallel, two ends of each cross shaft are supported through one group of the supports, the supports are fixed on the baseplate, each cross shaft is provided with the shaft sleeve, each fixing shaft is mounted in the corresponding shaft sleeve and capable of moving up and down along the vertical direction, the lower end of each fixing shaft is provided with a pressure pad, each fixing mechanism limits up-down movement of each fixing shaft along the vertical direction, and supports are arranged on the plate cover of each tissue culture plate.

Description

technical field [0001] The invention relates to a fixing device for a cell culture plate, in particular to a pressing device for a multilayer cell culture plate. Background technique [0002] With the development of molecular biology, the heterologous protein expression technology has gradually matured and been widely used. The expression of foreign proteins requires the selection of suitable expression hosts and expression elements, which requires the construction of a huge expression library, from which a better expression kit can be selected. The cell culture plate can use less culture medium for high-throughput cell culture and screening of strains, cell lines and expression libraries, and has the characteristics of fast, high-throughput, and cost-saving. [0003] In order to avoid bacterial contamination or cross-contamination between plate wells, the existing cell culture plates need to be sealed. There are currently two ways to seal the plate mouth, one is the latex ...

AI Technical Summary

Problems solved by technology

However, the seal cannot be guaranteed when the plate cover and well plate are used together, and a specific culture shaker is required

And when the orifice plate is cultured on the shaker, the size of the shaker plate limits the number of orifice plates that can be cultured at the same time

However, the three-dimensional space of the shaking table cannot be effectively utilized

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