Gluconobacter oxydans and application thereof
A technology of Gluconobacter oxydans and strains, which is applied in the field of agricultural biology and can solve problems such as insufficient safety and effectiveness
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Embodiment 1
[0035] Prepare the culture medium, the composition is the glucose of 20g / L, the potato of 50g / L and the yeast extract of 10g / L.
[0036] Gluconobacter oxydans with the preservation number CGMCC NO.8039 was inoculated and cultured in the above-mentioned medium until the cell concentration reached 10 9 CFU / mL to obtain bacterial agent.
[0037] A typical colony diagram of this strain is shown in figure 1 and figure 2 shown.
Embodiment 2
[0039]Use Gluconobacter oxydans with the preservation number CGMCC NO.8039 to treat Botrytis cinerea, Botrytis cinerea, Plasmopara uiticola (Berk.dt Curtis), grape powder Uncinula necator, Penicillium expansum, Botrytis cinerea, Penicillium expansum (Link) Thom, Gloeosporium piperatum, Fusarium oxysporum f.sp. Lycopersici Snyder et Hansen), Monilinia fructicola, Monilinia laxa, Monilinia fructigena, Monilinia fructicola, Aspergillus niger ( Aspergillus niger), P.digitatum (Fr.) Sall, Penicillium italicum, and Fusarium oxysporum, and Caenorhabditis elegans and / or southern root Knot nematode (Meloidogyne incognita) was tested for antibacterial effect, and the results shown in Table 1 were obtained.
[0040] Among them, postharvest peach brown rot fungus, apple postharvest gray mold fungus, and apple postharvest penicillium fungus were selected as indicator fungi, and plate confrontation culture method and fruit in vivo artificial punching inoculation method were adopted.
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