A method for plasma treatment of multi-walled carbon nanotube immobilized enzyme
A technology of multi-walled carbon nanotubes and plasma, which is applied in the direction of immobilization on or in the inorganic carrier, can solve the problems of cumbersome immobilization process, reduced enzyme activity, high cost, etc., and achieve the improvement of specific surface area and surface free energy, The process is simple and fast, and the effect of low operating cost
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Embodiment 1
[0031] Immobilization of porcine pancreatic lipase by plasma treatment of multi-walled carbon nanotubes
[0032] Step 1, place 0.1 g of multi-walled carbon nanotubes with a wall layer of 20 in a low-temperature vacuum plasma device, adjust the pulse width of the current to set the power at 74.8 W, and adjust the distance between the plasma electrodes to 4 cm, Treat at a vacuum of -99.99KPa for 10 min.
[0033] Step 2, transfer the treated multi-walled carbon nanotubes to a 50 ml Erlenmeyer flask, add 50 ml of phosphate buffer solution with a pH value of 7.0, and sonicate for 10 min. Weigh 0.15 g of porcine pancreatic lipase into the Erlenmeyer flask, put it into a constant temperature magnetic heating stirrer, set the temperature at 30 ° C, rotate at 200 rpm, and fix it for 1 h to obtain a mixed solution;
[0034] In step 3, the mixed solution is suction-filtered and washed with a large amount of phosphate buffer to remove unadsorbed free enzyme, and dried overnight at room t...
Embodiment 2
[0038] Immobilization of Lipozyme CALB L Lipase on Multi-walled Carbon Nanotubes by Plasma Treatment
[0039] Step 1, place 0.15 g of multi-walled carbon nanotubes in a low-temperature vacuum plasma device, adjust the pulse width of the current to set the power at 74.8 W, and adjust the distance between the two electrodes of the plasma to 4 cm. The vacuum degree is -99.00KPa for 4 minutes.
[0040]Step 2, put the treated multi-walled carbon nanotubes into a 50 ml Erlenmeyer flask, add 50 ml of phosphate buffer solution with a pH value of 7.0, and sonicate for 10 min. Add 3 ml of Lipozyme CALB L lipase into the Erlenmeyer flask, put it into a constant temperature magnetic heating stirrer, set the temperature at 30°C, rotate at 250 rpm, and fix it for 40 minutes to obtain a mixture;
[0041] In step 3, the mixed solution is suction-filtered and washed with a large amount of phosphate buffer to remove unadsorbed free enzyme, and dried overnight at room temperature to obtain lipa...
Embodiment 3
[0044] Immobilization of Lipozyme TL100 L lipase on multi-walled carbon nanotubes by plasma treatment
[0045] As described in Example 2, the difference is that Lipozyme TL100 L lipase enzyme solution is used as the free enzyme for immobilization. The enzyme activity of the immobilized enzyme with the plasma-treated multi-walled carbon nanotubes as the carrier is 146 U / g, and the enzyme protein loading capacity of the immobilized lipase per gram of multi-walled carbon nanotubes is 0.06g, which is higher than that of the unused The enzyme activity of the immobilized enzyme on the plasma treatment carrier increased by 19%, and the enzyme protein loading increased by 17%.
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