Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fibroin porous material loading insulin/fibroin microspheres and preparation method thereof

A porous material and insulin technology, applied in bandages, absorbent pads, medical science, etc., can solve the problems of cell toxicity and side effects, achieve high microsphere production, improve wound healing quality, and avoid toxic and side effects

Active Publication Date: 2015-03-25
SUZHOU UNIV
View PDF5 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results show that the material can effectively release insulin for 21 days, and the released insulin can promote the migration of keratinocytes, but organic solvents are introduced during the preparation of microspheres, which has potential cytotoxic side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fibroin porous material loading insulin/fibroin microspheres and preparation method thereof
  • Fibroin porous material loading insulin/fibroin microspheres and preparation method thereof
  • Fibroin porous material loading insulin/fibroin microspheres and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Put 150 g of silkworm silk into 6 L of 0.05% Na 2 CO 3 In aqueous solution, at 98~100 o C treatment for 30 min, repeated 3 times to degumming silk, fully washed and dried to obtain pure silk fiber. Add pure silk fiber to the ternary solution (the molar ratio of calcium chloride: water: ethanol is 1:8:2), at 72 o C stirring and dissolving into a mixed solution of silk fibroin. Put the obtained silk fibroin mixed solution into a dialysis bag, and dialyze with deionized water for 4 days to obtain a purified silk fibroin solution.

[0027] (2) Adjust the concentration of silk fibroin solution to 1 wt.%, add glycerin accounting for 30% of the mass of silk fibroin, and stir well to make the outer layer solution; dissolve insulin in 0.01 M HCl solution to make a concentration of 1 mg / mL of solution, adjusted its pH value to 6.5 with 0.1 M NaOH solution as the core layer solution. Microspheres were prepared by coaxial electrospinning technology, the power supply volta...

Embodiment 2

[0034] (1) Add the degummed silkworm silk fiber into 9.3 M LiBr solution, o C stirring and dissolving into a mixed solution of silk fibroin. Put the obtained silk fibroin mixed solution into a dialysis bag, and dialyze with deionized water for 4 days to obtain a purified silk fibroin solution.

[0035] (2) Adjust the concentration of silk fibroin solution to 5 wt.%, then add glycerin accounting for 50% of the mass of silk fibroin, and stir well to make the outer layer solution; dissolve insulin in 0.01 M HCl solution to make a concentration of 20 mg / mL solution, and then adjust its pH value to 6.5~7.2 with 0.1 M NaOH solution as the core layer solution. Microspheres were prepared by coaxial electrospinning technology, the power supply voltage was 15 kV, the injecting speed of insulin solution in the core layer was 1 mL / h, the injecting speed of silk fibroin solution in the shell layer was 1 mL / h, and the collection distance was 15 cm, the spun microspheres fell into a liqui...

Embodiment 3

[0038] (1) Put 100 g of tussah raw silk into 5 L of 0.25% Na 2 CO 3 In aqueous solution, at 98~100 o C treatment for 45 min, repeated 3 times to degumming silk, fully washed and dried to obtain tussah silk fiber. Add tussah silk fiber into molten calcium nitrate tetrahydrate, at 105 o C stirring and dissolving into tussah silk protein mixed solution. Put the obtained mixed solution into a dialysis bag, and dialyze with deionized water for 4 days to remove impurities to obtain a purified tussah silk fibroin protein solution.

[0039] (2) Adjust the concentration of silk fibroin solution to 2 wt.%, add glycerin accounting for 30% of the mass of silk fibroin, and stir evenly to make the outer layer solution; dissolve insulin in 0.01 M HCl solution to prepare a concentration of 10 mg / mL solution, adjust its pH value to 6.5-7.2 with 0.1 M NaOH solution as the core layer solution. The microspheres were prepared by coaxial electrospinning technology, the power supply voltage wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to View More

Abstract

The invention discloses a fibroin porous material loading insulin / fibroin microspheres and a preparation method thereof, belonging to the technical field of biomedical materials. The fibroin microspheres loading insulin are prepared by using a coaxial high voltage electrostatic technique and a freeze drying method and the porous material loading insulin / fibroin microspheres is prepared by using a layer-by-layer assembly and freeze drying method. The porosity of the porous material is 70-90% and the bore diameter is 50-200 microns. The microspheres are uniformly distributed inside the porous material and are firmly combined with the material. Insulin loaded in the material can be sustainably and slowly released for over 20 days and the releasing rate is over 94%. Insulin released from the fibroin porous material loading insulin / fibroin microspheres prepared by the invention has bioactivity and can stimulate epidermis cells and endothelial cells to migrate so as to increase the wound healing speed and improve the wound healing quality. The fibroin porous material loading insulin / fibroin microspheres disclosed by the invention provides wound dressing with a good performance for treating such defects of skins as wounds and burn and chronic ulcer of skins.

Description

technical field [0001] The invention relates to a silk fibroin porous material loaded with insulin / silk fibroin microspheres and a preparation method thereof. The material can be used for the treatment of wounds, burns and other skin defects and chronic skin ulcers, and belongs to the technical field of biomedical materials. Background technique [0002] The skin is the largest organ of the human body, it is not only the external barrier of the body but also a bridge for the body to communicate with the outside world. There are a large number of patients with skin defects every year in our country, mainly trauma, burns or chronic skin ulcers caused by diseases such as diabetes. In recent years, with the rapid increase in the number of diabetic patients, the problem of diabetic complication with refractory wounds has become an urgent clinical problem to be solved. Therefore, it is an urgent clinical need to develop biological dressings for difficult-to-heal wounds. As a "g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L15/32A61L15/44A61L15/42
Inventor 李秀芳李明忠尤仁传刘雨
Owner SUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com