Fibroin porous material loading insulin/fibroin microspheres and preparation method thereof
A porous material and insulin technology, applied in bandages, absorbent pads, medical science, etc., can solve the problems of cell toxicity and side effects, achieve high microsphere production, improve wound healing quality, and avoid toxic and side effects
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Embodiment 1
[0026] (1) Put 150 g of silkworm silk into 6 L of 0.05% Na 2 CO 3 In aqueous solution, at 98~100 o C treatment for 30 min, repeated 3 times to degumming silk, fully washed and dried to obtain pure silk fiber. Add pure silk fiber to the ternary solution (the molar ratio of calcium chloride: water: ethanol is 1:8:2), at 72 o C stirring and dissolving into a mixed solution of silk fibroin. Put the obtained silk fibroin mixed solution into a dialysis bag, and dialyze with deionized water for 4 days to obtain a purified silk fibroin solution.
[0027] (2) Adjust the concentration of silk fibroin solution to 1 wt.%, add glycerin accounting for 30% of the mass of silk fibroin, and stir well to make the outer layer solution; dissolve insulin in 0.01 M HCl solution to make a concentration of 1 mg / mL of solution, adjusted its pH value to 6.5 with 0.1 M NaOH solution as the core layer solution. Microspheres were prepared by coaxial electrospinning technology, the power supply volta...
Embodiment 2
[0034] (1) Add the degummed silkworm silk fiber into 9.3 M LiBr solution, o C stirring and dissolving into a mixed solution of silk fibroin. Put the obtained silk fibroin mixed solution into a dialysis bag, and dialyze with deionized water for 4 days to obtain a purified silk fibroin solution.
[0035] (2) Adjust the concentration of silk fibroin solution to 5 wt.%, then add glycerin accounting for 50% of the mass of silk fibroin, and stir well to make the outer layer solution; dissolve insulin in 0.01 M HCl solution to make a concentration of 20 mg / mL solution, and then adjust its pH value to 6.5~7.2 with 0.1 M NaOH solution as the core layer solution. Microspheres were prepared by coaxial electrospinning technology, the power supply voltage was 15 kV, the injecting speed of insulin solution in the core layer was 1 mL / h, the injecting speed of silk fibroin solution in the shell layer was 1 mL / h, and the collection distance was 15 cm, the spun microspheres fell into a liqui...
Embodiment 3
[0038] (1) Put 100 g of tussah raw silk into 5 L of 0.25% Na 2 CO 3 In aqueous solution, at 98~100 o C treatment for 45 min, repeated 3 times to degumming silk, fully washed and dried to obtain tussah silk fiber. Add tussah silk fiber into molten calcium nitrate tetrahydrate, at 105 o C stirring and dissolving into tussah silk protein mixed solution. Put the obtained mixed solution into a dialysis bag, and dialyze with deionized water for 4 days to remove impurities to obtain a purified tussah silk fibroin protein solution.
[0039] (2) Adjust the concentration of silk fibroin solution to 2 wt.%, add glycerin accounting for 30% of the mass of silk fibroin, and stir evenly to make the outer layer solution; dissolve insulin in 0.01 M HCl solution to prepare a concentration of 10 mg / mL solution, adjust its pH value to 6.5-7.2 with 0.1 M NaOH solution as the core layer solution. The microspheres were prepared by coaxial electrospinning technology, the power supply voltage wa...
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