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A kind of silk fibroin porous material loaded with insulin/silk fibroin microspheres and its preparation method

A porous material and insulin technology, applied in bandages, absorbent pads, medical science, etc., can solve problems such as cytotoxic side effects, and achieve the effects of high yield of microspheres, accelerated wound healing, and simple equipment.

Active Publication Date: 2017-09-22
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results show that the material can effectively release insulin for 21 days, and the released insulin can promote the migration of keratinocytes, but organic solvents are introduced during the preparation of microspheres, which has potential cytotoxic side effects

Method used

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  • A kind of silk fibroin porous material loaded with insulin/silk fibroin microspheres and its preparation method
  • A kind of silk fibroin porous material loaded with insulin/silk fibroin microspheres and its preparation method
  • A kind of silk fibroin porous material loaded with insulin/silk fibroin microspheres and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Put 150 g of silkworm silk into 6 L of 0.05% Na 2 CO 3 In aqueous solution, at 98~100 o C treatment for 30 min, repeated 3 times to degumming silk, fully washed and dried to obtain pure silk fiber. Add pure silk fiber to the ternary solution (the molar ratio of calcium chloride: water: ethanol is 1:8:2), at 72 o C stirring and dissolving into a mixed solution of silk fibroin. Put the obtained silk fibroin mixed solution into a dialysis bag, and dialyze with deionized water for 4 days to obtain a purified silk fibroin solution.

[0027] (2) Adjust the concentration of silk fibroin solution to 1 wt.%, add glycerin accounting for 30% of the mass of silk fibroin, and stir well to make the outer layer solution; dissolve insulin in 0.01 M HCl solution to make a concentration of 1 mg / mL of solution, adjusted its pH value to 6.5 with 0.1 M NaOH solution as the core layer solution. The microspheres were prepared by coaxial electrospinning technology, the power supply v...

Embodiment 2

[0034] (1) Add the degummed silkworm silk fiber into 9.3 M LiBr solution, o C stirring and dissolving into a mixed solution of silk fibroin. Put the obtained silk fibroin mixed solution into a dialysis bag, and dialyze with deionized water for 4 days to obtain a purified silk fibroin solution.

[0035] (2) Adjust the concentration of silk fibroin solution to 5 wt.%, then add glycerin accounting for 50% of the mass of silk fibroin, and stir well to make the outer layer solution; dissolve insulin in 0.01 M HCl solution to make a concentration of 20 mg / mL solution, and then use 0.1M NaOH solution to adjust its pH value to 6.5~7.2 as the core layer solution. Microspheres were prepared by coaxial electrospinning technology, the power supply voltage was 15 kV, the injecting speed of insulin solution in the core layer was 1 mL / h, the injecting speed of silk fibroin solution in the shell layer was 1 mL / h, and the collection distance was 15 cm, the spun microspheres fell into a liqu...

Embodiment 3

[0038] (1) Put 100 g of tussah raw silk into 5 L of 0.25% Na 2 CO 3 In aqueous solution, at 98~100 o C treatment for 45 min, repeated 3 times to degumming silk, fully washed and dried to obtain tussah silk fiber. Add tussah silk fiber into molten calcium nitrate tetrahydrate, at 105 o C stirring and dissolving into tussah silk protein mixed solution. Put the obtained mixed solution into a dialysis bag, and dialyze with deionized water for 4 days to remove impurities to obtain a purified tussah silk fibroin protein solution.

[0039] (2) Adjust the concentration of silk fibroin solution to 2 wt.%, add glycerin accounting for 30% of the mass of silk fibroin, and stir evenly to make the outer layer solution; dissolve insulin in 0.01 M HCl solution to prepare a concentration of 10 mg / mL solution, adjust its pH value to 6.5-7.2 with 0.1 M NaOH solution as the core layer solution. The microspheres were prepared by coaxial electrospinning technology, the power supply voltage wa...

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Abstract

The invention discloses a silk fibroin porous material loaded with insulin / silk fibroin microspheres and a preparation method thereof, belonging to the technical field of biomedical materials. Insulin-loaded silk fibroin microspheres were prepared by coaxial high-voltage electrostatic technology and freeze-drying method, and porous materials loaded with insulin / silk fibroin microspheres were prepared by layer-by-layer assembly and freeze-drying methods. The porosity of the porous material was 70%~ 95%, the pore size is 50-200μm, the microspheres are evenly distributed inside the porous material, and are firmly combined with the material. The insulin loaded in the material can be released slowly for more than 20 days, and the release rate reaches more than 94%. The insulin released by the porous silk material loaded with insulin / silk fibroin microspheres prepared by the method has biological activity and can stimulate the migration of epidermal cells and endothelial cells to promote the speed of wound healing and improve the quality of wound healing. The invention provides a wound dressing with good performance for the treatment of wounds, burns and other skin defects and chronic skin ulcers.

Description

technical field [0001] The invention relates to a silk fibroin porous material loaded with insulin / silk fibroin microspheres and a preparation method thereof. The material can be used for the treatment of wounds, burns and other skin defects and chronic skin ulcers, and belongs to the technical field of biomedical materials. Background technique [0002] The skin is the largest organ of the human body, it is not only the external barrier of the body but also a bridge for the body to communicate with the outside world. There are a large number of patients with skin defects every year in our country, mainly trauma, burns or chronic skin ulcers caused by diseases such as diabetes. In recent years, with the rapid increase in the number of diabetic patients, the problem of diabetic complication with refractory wounds has become an urgent clinical problem to be solved. Therefore, it is an urgent clinical need to develop biological dressings for difficult-to-heal wounds. As a "gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32A61L15/44A61L15/42
Inventor 李秀芳李明忠尤仁传刘雨
Owner SUZHOU UNIV
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