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Application of a thermostable carbonic anhydrase in catalyzing the desorption of CO2 from organic amine solution

A carbonic anhydrase enzyme solution, thermal stability technology, applied in the directions of lyase, chemical instruments and methods, biosynthesis, etc., to achieve the effects of reducing energy consumption, saving energy, and improving MDEA tolerance

Active Publication Date: 2016-03-23
LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, carbonic anhydrase has catalyzed the absorption of CO by organic amines. 2 In the process, many researches and applications have been obtained, while domestic and foreign studies on carbonic anhydrase catalyzed CO 2 Desorption methods are still rarely reported

Method used

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  • Application of a thermostable carbonic anhydrase in catalyzing the desorption of CO2 from organic amine solution
  • Application of a thermostable carbonic anhydrase in catalyzing the desorption of CO2 from organic amine solution
  • Application of a thermostable carbonic anhydrase in catalyzing the desorption of CO2 from organic amine solution

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, the preparation of PaCA enzyme liquid

[0035] 1.1 Medium:

[0036] LB solid medium (g / L): yeast extract 5, peptone 10, sodium chloride 10, agar 15

[0037] LB liquid medium (g / L): yeast extract 5, peptone 10, sodium chloride 10

[0038] 1.2 Preparation steps

[0039] The carbonic anhydrase nucleotide sequence (SeqNo.1, named PaCA) was obtained according to the Blast comparison. After optimization, the whole gene was synthesized by Nanjing GenScript Biotechnology Co., Ltd., and then ligated with the plasmid pET21a (Novagen) for recombination Plasmid PaCA-pET21a was transformed into BL21(DE3) competent cells (purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.), and a single colony of positive clones was picked for expression verification of soluble active expression.

[0040] Streak the Escherichia coli in the glycerol tube on the plate with ampicillin-resistant LB solid medium. Cultivate at 37°C for 12-16h, pick a single colony and trans...

Embodiment 2

[0043] Materials and instruments: The reactor is a 5L jacketed glass reactor (height-to-diameter ratio: 8:1), provided by Shanghai Kexing Glass Instrument Factory. N-methyldiethanolamine (MDEA), analytically pure; carbonic anhydrase, self-made (PaCA); CO 2 Gas was provided by Shanghai Xiqin Gas Co., Ltd.

[0044] 1. PaCA promotes MDEA desorption of CO at 60°C 2

[0045] Prepare 2L3M MDEA solution and absorb CO in a 5L reactor at room temperature 2 , the absorption time is 60min, CO 2 The infusion rate was 6L / min. After the absorption, the MDEA solution was divided into two parts, one part was added with 800U / mLPaCA enzyme solution, and one part was used as a blank control, and the desorption experiment was carried out at 60°C, and the pH change of the system was detected online during the reaction. CO measured from within 10min 2 The change of pH value during the desorption process ( figure 1 ) shows that, compared with the control, the CO after adding the enzyme 2 The...

Embodiment 3

[0053] Example 3: PaCA promotes CO desorption from MEA at 60°C 2

[0054] MEA desorption at 60°C, concentration and other conditions are the same as in Example 2

[0055] Prepare 2L3M MEA solution and absorb CO at room temperature in a 5L reactor 2 , the absorption time is 40min, CO 2 The infusion rate was 6L / min. After the absorption, the MDEA solution was divided into two parts, one part was added with 900U / mLPaCA enzyme solution, and one part was used as a blank control, and the desorption experiment was carried out at 60°C, and the pH change of the system was detected online during the reaction. CO measured from within 10min 2 pH value of desorption process, compared with control, CO after enzyme addition 2 The desorption rate is relatively high.

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Abstract

The invention belongs to the field of CO2 emission reduction treatment and provides application of thermal stability carbonic anhydrase in catalysis of organic amine solution and desorption of CO2. The thermal stability carbonic anhydrase is an amino acid sequence shown as SEQ ID NO:2. The carbonic anhydrase (PaCA) expressed by gene modification in a lab is adopted for catalyzing CO2 enriched organic amine solution desorption at different temperatures, and the carbonic anhydrase can effectively promote the desorption rate of CO2 at different low desorption temperatures. Compared with a mode with high needed chemical desorption temperature, the application has the advantages that the enzyme catalysis efficiency is high, the desorption temperature can be reduced, the energy consumption is reduced, and the energy is saved.

Description

technical field [0001] The invention belongs to CO 2 The field of emission reduction treatment, specifically relates to a kind of thermostable carbonic anhydrase in catalyzing organic amine solution to desorb CO 2 in the application. Background technique [0002] Carbon dioxide is one of the main gases that cause the greenhouse effect, and with the continuous development of the national economy, the emission of carbon dioxide is also increasing. In response to concerns about global climate change, carbon dioxide emission reduction strategies have received extensive attention internationally, and have become a research and development hotspot in the international energy field. my country's carbon dioxide emissions rank first in the world, and the pressure to reduce carbon dioxide emissions is increasing, which will become one of the bottlenecks restricting the sustainable development of coal-fired power generation in my country. [0003] The capture and utilization of carb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/88B01D53/86B01D53/62B01D53/96
CPCY02P20/59
Inventor 祝俊余允东周晓青张燕汪浩龙辉张敏陈亭亭陈风义
Owner LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
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