A kind of method for identifying species of insects of the genus Phytophthora
A technology for species identification and gypsy moths, which is applied in the field of species identification of gypsy moths, can solve the problems of increased difficulty in inspection and quarantine of gypsy moths at ports, and difficulty in accurate identification by morphological methods, achieving high accuracy, easy operation, and simple methods Effect
Inactive Publication Date: 2017-03-29
CHANGZHOU ENTRY EXIT INSPECTION & QUARANTINE +2
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Problems solved by technology
Larvae and eggs are often found on ships, and it is difficult to accurately identify them with morphological methods, which increases the difficulty of inspection and quarantine of gypsy moths at ports
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Embodiment 1
[0022] The insect that will be determined as the genus of Phytophthora adopts the method of the present invention to identify species, specifically as follows:
[0023] 1. Download the mitochondrial COI gene sequences of Lymantria insects from the BOLD (http: / / www.boldsystems.org / ) public database. There are 17 species and 524 sequences. Clustal W software in the MEGA software package was used for multiple sequence alignment, that is, all sequences were aligned. All sequences were converted to 583bp long sequences. Find out the difference sites of each species of the genus Phylophaga, and form the standard sequence of each species of the genus; the standard sequence retains all the difference sites of each species, and other sites are complemented with "N" so that the length is 583bp; each Species difference sites refer to the base sites where the mitochondrial COI gene sequence is conserved within a species and differs between species, such as Figure 1-5 shown. Conservati...
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The invention provides a species identification method of euproctis insects and relates to the field of biotechnology. The method provided by the invention comprises the following steps: finding different sites of a mitochondria COI gene sequence of euproctis in each species to form the standard sequence of euproctis of each species; comparing the mitochondria COI gene sequence of a to-be-detected sample of euproctis with the standard sequence of each species and taking the maximum similarity value, wherein the to-be-tested insect belongs to the species corresponding to the maximum value. The method provided by the invention can be used for identifying insects of euproctis, is simple to operate, high in accuracy, short in time consumption and high in specificity and provides technical support for identification of euproctis insects. By using a different site rule of the mitochondria COI gene sequence as an identification characteristic of the species, an identity card is established for species so as to accurately differentiate allied species. According to the method provided by the invention, sub sequences of the to-be-tested sequence are sequentially compared with a standard sequence and scored, so that the method is more reasonable, accurate and rigorous.
Description
technical field [0001] The invention relates to the field of biological technology, in particular to a method for identifying species of insects of the genus Phytophthora. Background technique [0002] Lymantria dispar (abbreviated as L.dispar) belongs to the genus Lymantria of the family Lymantriidae in the order Lepidoptera, and is an omnivorous agricultural and forestry pest. Based on geographic distribution, biological learning, and morphological characteristics, the gypsy moth (L. dispar) is now divided into the Asian subspecies (L. dispar asiatica Vnukovskii), the European subspecies (L. dispar dispar Linnaeus), and the Japanese subspecies (L. dispar japonica Motschulsky). [0003] Because the Asian gypsy moth adults have the ability to fly and have a larger host range than the European type, the relevant departments of the United States believe that the Asian gypsy moth is more threatening than the European type. In order to prevent the Asian gypsy moth from being in...
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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 钱路安榆林宋俊贤叶兼菱郝德君徐梅杨晓军伏建国吴晶郑斯竹
Owner CHANGZHOU ENTRY EXIT INSPECTION & QUARANTINE