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Method and device for rapid detection of amplified nucleotide sequences

A target nucleotide sequence and sequence technology, which is applied in the field of nucleotide sequences and equipment for rapid detection and amplification, and can solve problems such as complex equipment, interference detection, and high cost

Inactive Publication Date: 2015-04-08
CORIS BIOCONCEPT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such methods and equipment for measurement and analysis are often complex and expensive
Further, microbubble emissions generated during the primary (gene) amplification step can interfere with detection on these devices

Method used

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  • Method and device for rapid detection of amplified nucleotide sequences
  • Method and device for rapid detection of amplified nucleotide sequences
  • Method and device for rapid detection of amplified nucleotide sequences

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Embodiment Construction

[0050] Such as figure 1 The described equipment includes means for microfluidic handling of samples, allowing rapid and specific (gene) amplification of (nucleic acid) sequences, in particular PCR-type amplification with constant (or continuous) dynamic flow, combined for detection These are devices that amplify nucleotide sequences (amplicons).

[0051] Such as figure 1 As shown, the apparatus of the present invention comprises a first (gene) amplification region or chamber 1 comprising different suitable means for carrying out such (gene) amplification by PCR, and comprising means for carrying out The second region or detection chamber 2 of the different suitable means for such detection of amplified gene sequences.

[0052] The device as described in chamber 1 is known from the prior art and may comprise different means for (gene) amplification in microfluidics and preferably under constant dynamic flow, preferably by PCR.

[0053] These devices comprise channels 3 manuf...

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Abstract

The present invention relates to a method and a device for amplification and rapid detection of target nucleotide sequences possibly present in a sample, combining continuous-flow PCR amplification with oligochromatography detection by capillary action on a test strip.

Description

technical field [0001] The present invention relates to methods and devices for the rapid detection of genetic sequences (amplicons) from the (genetic) (enzymatic) amplification of original and specific genetic sequences present in biological samples. [0002] The method is preferably designed for the diagnostic application of samples comprising different multiple gene target sequences (oligonucleotides), (i) for the rapid and efficient detection of a specific nucleic acid sequence in a sample, and for the detection and the test sample The association of different pathologies and diseases of individuals from which, in particular infectious diseases, bacteremia, respiratory or intestinal infectious diseases, including forms of resistance to therapeutic agents such as antibiotics, (ii) identification of food Contaminants, or (iii) for prognostic testing, especially detection of cancer markers that may be present in the sample. Background technique [0003] Among the (gene) am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/686B01L3/502715B01L7/525B01L3/50273B01L2200/10B01L2300/0816B01L2300/069B01L2300/0825B01L2300/0636B01L2300/1805B01L2300/088B01L2400/0481G01N2021/6439C12Q2537/143C12Q2565/625C12Q2565/629B01L7/52C12Q1/689C12Q2563/107C12Q2527/113B01L2300/1822B01L2300/1827
Inventor 蒂埃里·勒克利普特帕斯卡尔·默腾斯利蒂希娅·阿夫兰伊莎贝尔·奥特瓦莱里·斯米肯斯
Owner CORIS BIOCONCEPT
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