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A kind of caffeine extract of mutable goat grass and its extraction method and application

A technology of volatile goat grass and an extraction method, which is applied in the field of caffeine extract and its extraction, and achieves the effects of less dosage, high product purity and simple operation.

Inactive Publication Date: 2017-08-08
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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Problems solved by technology

[0005] At present, domestic research has not reported caffeine metabolism related to the resistance of gramineous plants to pathogenic nematodes. With the development of transcriptome sequencing technology in recent years, there have been reports on the use of transcriptome technology combined with comparative genome technology to study plant resistance. growing year by year

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  • A kind of caffeine extract of mutable goat grass and its extraction method and application
  • A kind of caffeine extract of mutable goat grass and its extraction method and application
  • A kind of caffeine extract of mutable goat grass and its extraction method and application

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Embodiment 1

[0028] 1. Experimental materials and reagents

[0029]1. Sequenced plant material: Select Goatweed variabilis (MQ Yu et al (1990) Agronomie, 6, 451-456) with CCN resistance and RKN resistance as the transcriptome sequencing material to determine its effect on inoculation of CCN treatment and non-inoculation The expression of all genes in roots under two conditions of CCN. First, the seeds of the material are subjected to surface sterilization treatment (soaked in the mixed solution of 3% sodium hypochlorite and 0.01% Tween20 for 5 minutes and then soaked 3 times with sterilized water, and then the seeds are evenly spread on the continuously moist filter paper and placed In a petri dish with a diameter of 5 cm, seedlings were germinated at a room temperature of about 20°C and a photoperiod of 16h / 8h. Ten days later, the seedlings were divided into two groups: a group of J2 larvae (H.avenae) for inoculation with CCN ( Each plant was inoculated with 1000), another group was not ...

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Abstract

The invention discloses a caffeine extract of goat grass vulgaris and its extraction method and application, belonging to the field of bioengineering. The specific steps of the extraction method of the caffeine extract of Goatweed vulgaris in the present invention are as follows: take the root of Goatweed vulgaris, wash it, cut it into slices, dry it in an oven at 80°C for 6 hours to remove moisture thoroughly, and then pulverize it with a pulverizer , after passing through a 60-mesh sieve, put 10 grams of pulverized goat grass root samples into a 50-milliliter extraction kettle, and use absolute ethanol as an entrainer. 2 30MPa in the extractor, extract at 60°C for 2h, in which CO 2 The flow rate is controlled at 1.5LPM to obtain the caffeine extract of mutable goat grass. The research of the present invention shows that >0.1% caffeine has a growth inhibitory effect on plant pathogenic nematodes, and the caffeine extract of goat grass vulgaris obtained in the present invention can be used to prepare insect-resistant insecticides for cereal cyst nematodes and root-knot nematodes incognita agent.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to a caffeine extract of goat grass vulgaris and its extraction method and application. Background technique [0002] Caffeine (Caffeine, caffeine) is a xanthine alkaloid, and there are two common types of caffeine water and caffeine anhydrous. Caffeine is a stimulant for the central nervous system. Caffeine is mainly found in certain kinds of plants and is an alkaloid. By paralyzing insects that invade plants, it acts as an insecticide, protecting plants from pests and diseases. At first, people in the world mainly obtained caffeine from coffee beans, and its content varied according to different types of coffee beans and different production processes. It was later discovered that tea also contains caffeine, and the amount of caffeine contained in a tea usually depends on the strength of the tea made. Therefore, the caffeine content in green tea, black tea, and Pu'er tea is diffe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/44A01P5/00
Inventor 余懋群郑明辉李林龙海徐德林潘志芬邓光兵
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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