Amylase modified product for co-delivery of genes and antitumor drugs as well as preparation method and application thereof

An anti-tumor drug, amylose technology, applied in the field of biomedical engineering materials, to achieve selectivity, reduce product cytotoxicity, and improve water solubility

Active Publication Date: 2015-04-22
GUANGZHOU REFORGENE MEDICINE CO LTD
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there has been no research involving the inclusion of amylose or its modified substances to hydrophobic anti-tumor drug molecules and their combined delivery with genes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Amylase modified product for co-delivery of genes and antitumor drugs as well as preparation method and application thereof
  • Amylase modified product for co-delivery of genes and antitumor drugs as well as preparation method and application thereof
  • Amylase modified product for co-delivery of genes and antitumor drugs as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Mix 0.48g of dry amylose and 8mL of 1-azido-propylene oxide and dissolve in 20mL of aqueous sodium hydroxide (aqueous solution of sodium hydroxide formed by adding 0.4g of sodium hydroxide to 100ml of water), at 30°C Stir in the dark for 24 hours; after the reaction, dialyze the reaction product with a 14,000 cut-off dialysis bag for 48 hours, filter, and freeze-dry to obtain azide-containing amylose with a yield of 85%.

Embodiment 2

[0039] Mix 0.48g of dry amylose and 4mL of 1-azido-propylene oxide and dissolve in 20mL of aqueous sodium hydroxide solution (aqueous sodium hydroxide solution formed by adding 0.8g of sodium hydroxide to 100ml of water), at 40°C Stir in the dark for 36 hours; after the reaction, the reaction product was dialyzed for 72 hours, filtered, and freeze-dried to obtain amylose containing azide groups with a yield of 81%.

Embodiment 3

[0041] Mix 0.50g of the 3rd generation alkyne-containing dendritic polylysine with 50mg of the azide-group-containing amylose obtained in Example 1 and dissolve in 30mL of anhydrous dimethyl sulfoxide; 2 Protect for 15 minutes, then add 33.6mg copper sulfate pentahydrate and 53.2mg sodium ascorbate, heat up to 50°C and react for 48 hours; Amylose Modifications for Co-delivery of Antineoplastic Drugs. The yield was 79%, and the result of elemental analysis showed that the dendritic amylose derivative contained 9.05% N.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The invention belongs to the field of bio-medical engineering materials and discloses an amylase modified product for co-delivery of genes and antitumor drugs as well as a preparation method and an application of the amylase modified product in preparation of a co-delivery substance of genes and antitumor drugs. The preparation method comprises the following steps: azidating amylase and carrying out click reaction on azidated amylase and alkyne group-containing dendric polylysine to synthesize the amylase modified product. The amylase modified product is applied by the steps of by virtue of magnetic stirring and ultrasonic treatment, coating the hydrophobic anticancer drugs with the amylase modified product and carrying out electrostatic compounding on a large number of amino groups on side chains of dendric polylysine and plasmid DNA. The preparation method has the advantages of mild reaction conditions and efficient reaction, is easy to operate and the like and the amylase modified product is expected to play potential applications in the biomedical field.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials, and specifically relates to an amylose modified product used for co-delivery of genes and antitumor drugs, a preparation method and application thereof. Background technique [0002] In recent years, non-viral gene delivery vectors based on water-soluble cationic polymers have developed rapidly, and have become a frontier research field and focus of biomedical polymers. At present, the most important development trend of cationic polymer gene carriers is to carry out gene and drug co-delivery and achieve synergistic growth by simultaneously loading plasmid DNA and mostly hydrophobic anti-tumor drug molecules through polymer chemical structure change and performance regulation. effective treatment (Wang et al. Nature Materials, 2006, 5:791–796; Hyun, et al. Biomaterials, 2011, 32:306–315). However, the carriers used so far for co-delivery of genes and drugs are almost entirely limi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00A61K47/36A61K48/00C12N15/87C12N15/85
Inventor 张黎明庞家栋庄宝雄王捷
Owner GUANGZHOU REFORGENE MEDICINE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap