A rapid and sensitive method for the detection of Plasmodium malariae
A technology for sensitive detection of Plasmodium, applied in the field of in vitro diagnostic reagents, can solve problems such as complex operation, and achieve the effect of improving detection ability, reducing time and complexity
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[0037] Example 1 Specificity and sensitivity of detection of Plasmodium vivax
[0038] 1. Experimental steps
[0039] 1. Collection of blood samples
[0040] Take 1.0ml of the whole blood sample of the patient tested at the port.
[0041] 2. Preliminary identification of blood samples
[0042] The colloidal gold method was used to preliminarily determine whether the patient was infected with Plasmodium vivax.
[0043] 3. Extraction of genomic DNA
[0044] Use blood sample genomic DNA extraction kit (spin column type, Qiagen company) to extract the malaria vivax DNA. Take a 50uL whole blood sample and extract DNA according to the extraction steps in the kit instructions.
[0045] 4. Determination of DNA concentration
[0046] The DNA concentration of Plasmodium was quantified by real-time quantitative PCR using ABI's real-time fluorescence detector.
[0047] 5. Amplification of target genes
[0048] 5.1 Design of primers and probes
[0049] For the unique genes of Plasmodium vivax, design prim...
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