A kit for detecting drug-resistant genes of Mycobacterium tuberculosis and its application

Abstract

The invention discloses a kit for detecting mycobacterium tuberculosis drug resistance gene and application of the kit. The invention discloses a probe set which can be used for detecting or assisting detection that whether resistance mutation is produced or not in a mycobacterium tuberculosis gene of a sample, wherein the probe set comprises at least one of the following probes any shown in sequences (1)-(20): (1) a sequence shown in SEQ ID No.13; (2) a sequence shown in SEQ ID No.14; (3) SEQ ID No.15; and (4) a sequence shown in SEQ ID No.16. The probe set disclosed by the invention has the advantages that a high-efficiency, sensitive and multiple real-time fluorescent PCR technology, mutation condition of seven drug resistant genes of mycobacterium tuberculosis in a to-be-detected sample can be detected in a short time, result sensitivity is high, specificity is good, and an analysis process is simple and convenient.

Description

technical field [0001] The invention relates to a kit for detecting drug-resistant genes of mycobacterium tuberculosis and an application thereof, belonging to the field of biotechnology. Background technique [0002] Tuberculosis is the infectious disease with the highest mortality rate caused by a single bacterial infection. At present, about 1 / 3 of the people in the world are infected with Mycobacterium tuberculosis, and the number of people who die from tuberculosis every year reaches 3 million. my country is one of the 22 countries with severe tuberculosis epidemics in the world, ranking second in the world. The fifth national tuberculosis epidemiological sampling survey showed that the prevalence rate of tuberculosis among people aged 15 and over was 459 / 100,000, and the multidrug resistance rate of tuberculosis patients was 6.8%. The co-occurrence of tuberculosis and AIDS and other diseases, as well as the phenomenon of multidrug resistance, have made the prevention...

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Problems solved by technology

[0012] Capreomycin resistance is closely related to related gene mutations, including 16S rRNA gene and tlyA gene, however, the most important drug resistance gene is 16S rRNA gene, and about 78-80% of capreomycin resistance is related to the mutation of this gene Related, especially the single base substitution of 1401(A→G), the mutation rate is about 72%, but this mutant only causes capreomycin low resistance, 1402(C→T) and 1484(G→T) The mutation rate in serotonin is low, about 5-6%, but they can lead to strains that are highly resistant to capreomycin

[0013] The clinical detection of Mycobacterium tuberculosis has always relied mainly on drug susceptibility testing, but the traditional drug susceptibility testing on solid medium has a long period and low sensitivity, which seriously affects the early diagnosis and treatment of patients.

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