Content detection method of gamithromycin

A detection method and technology of gamiycin, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of low precision and poor separation effect, and achieve a large detection range, stable baseline, and good separation effect. Effect
CN104535693AActive Publication Date: 2015-04-22TIANJIN ZHONGSHENG TIAOZHAN BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN ZHONGSHENG TIAOZHAN BIOTECH
Publication Date
2015-04-22

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Abstract

The invention provides a content detection method of gamithromycin. According to the technical scheme, the content of gamithromycin is detected by virtue of a HPLC method; an evaporative light-scattering detector is utilized, is high in sensitivity and is not sensitive to temperature variation, and a base line is relatively stable; the response of the evaporative light-scattering detector does not depend on the optical property of a sample, is not influenced by functional groups, is not interfered by a mobile phase and is in direct proportion to the quality of the sample, and specific chemical structures of detected main components are not required; the gamithromycin is relatively low in absorption wavelength and is absorbed at a tail end of an ultraviolet ray; therefore, compared with an ultraviolet detector, the evaporative light-scattering detector has great superiority in the detection of gamithromycin. Besides, selected chromatographic columns and chromatographic conditions are designed tightly around the characteristic of the evaporative light-scattering detector, a relatively good separation effect is integrally realized; after the method is verified by virtue of methods such as an accuracy experiment, a linear relation experiment and a sampling recycling experiment, results show that the accuracy is high, the detection range is wide, and the sensitivity is relatively good.
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Description

technical field

[0001] The invention relates to the technical field of drug analysis, in particular to a method for detecting the content of gamycin. Background technique

[0002] Gamithromycin, English name: Gamithromycin, CAS number is 145435-72-9, molecular formula is C 40 H 76 N 2 O 12 , molecular weight 777.04, white to beige crystals, soluble in methanol, acetonitrile, acetone and other organic solvents, insoluble in water.

[0003] Gamycin is a semisynthetic macrolide veterinary antibiotic with only one stable configuration. Gamitomycin has bacteriostatic and bactericidal activities by inhibiting the synthesis of bacterial proteins. It is mainly used for the treatment of bovine infectious diseases caused by bacterial pathogens such as Pasteurella hemolytica, Pasteurella hemorrhagicus, and Histophilus . Gamiycin can be used in edible animals, and has the advantages of fast absorption, wide distribution in the body, low residual in the body, and high safety. No ad...

Claims

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