A kind of bee pollen extract and its preparation and application
A bee pollen and extract technology, applied in the fields of food, biochemical industry, and pharmaceuticals, can solve the problems of single active ingredient of the extract, inability to reflect the full effect of bee pollen, and large dosage required, so as to achieve controllable residue and good application prospects , the effect of reducing the effective dose
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Embodiment 1
[0021] One, the preparation method of bee pollen extract
[0022] Rapeseed pollen was used as raw material to prepare bee pollen extract according to the following process:
[0023] Take 1000 grams of dried bee pollen, add 5L of ethanol with a volume concentration of 70%, and extract at 20°C for 10 hours. After the extraction is completed, filter and separate the extract from the solids for processing:
[0024] (1) The treatment method of the extract: the extract is concentrated and dried in vacuum, the concentrated sample is dissolved in ethanol with a volume concentration of 70%, and then put on a macroporous resin column, first rinsed with deionized water of 5 times the column volume, and then with a volume concentration of The column was washed with 70% ethanol and fractions were collected, and the fraction washed with 70% ethanol was concentrated and dried in vacuo to obtain fraction 1.
[0025] (2) Solid matter treatment method: add water to the solid matter according t...
Embodiment 2
[0095] Using tea bee pollen as raw material, the bee pollen extract is prepared according to the following process:
[0096] Take 1,000 grams of dried tea bee pollen, add 50 L of ethyl acetate, and extract at 50°C for 2 hours. After the extraction is completed, filter and separate the extract from the solids for processing:
[0097] (1) The treatment method of the extract: the extract is concentrated and dried in vacuum, the concentrated sample is dissolved in 70% ethanol and then put on a macroporous resin column, first rinsed with deionized water of 5 times the column volume, and then washed with 70% ethanol The column was collected and fractions were collected, and the fraction washed with 70% ethanol was concentrated and dried in vacuo to obtain fraction 1.
[0098] (2) Solid matter treatment method: solid matter is added 0.01M Tris-HCl buffer solution according to the ratio of raw material: solution = 1kg: 2L, the pH of the mixed solution is adjusted to be in the range of...
Embodiment 3
[0102] Using corn bee pollen as raw material, the corn bee pollen extract is prepared according to the following process:
[0103] Take 1,000 grams of dried corn bee pollen, add 20 L of n-hexane, and extract at 30°C for 5 hours. After the extraction, filter and separate the extract from the solids for processing:
[0104] (1) The treatment method of the extract: the extract is concentrated and dried in vacuum, the concentrated sample is dissolved in 70% ethanol and then put on a macroporous resin column, first rinsed with 10 times the column volume of deionized water, and then washed with 50% ethanol The column was collected and the fractions were collected. The fractions washed with 50% ethanol were combined and then concentrated and dried in vacuo to obtain Fraction 1.
[0105] (2) Solid matter treatment method: solid matter is added with 0.05M phosphate buffer solution according to the ratio of raw material: solution = 1kg:5L, the pH of the mixed solution is adjusted to 7, ...
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