A kind of Pichia sp.SIT2014 and its culture method and application

Abstract

The invention discloses a Pichia sp. SIT2014, a cultivation method and an application thereof. The Pichia sp. SIT2014 has a preservation number of CGMCC NO.9300. Using it as a biocatalyst to catalyze the reduction of precursor 2-hydroxyarylethanone to chiral aryl-o-diol, the Pichia sp.SIT2014 cells were suspended in phosphate buffer and 2- For hydroxyaryl ethyl ketone, the asymmetric reduction reaction is carried out at a temperature of 30-50°C for 3-48 hours, the resulting reaction solution is centrifuged, and the supernatant is extracted and dried to obtain chiral aryl vicinal diol. Using this Pichia sp.SIT2014 as a biocatalyst to prepare chiral aryl vicinal diols, a variety of chiral aryl vicinal diols can be synthesized, and the conversion rate and enantiomeric excess are above 99%. .

Description

technical field [0001] The present invention relates to a yeast and its application, more specifically to a Pichiasp. Background technique [0002] Chiral compounds play an important role in human life. Since the two enantiomers of the same compound have huge differences in pharmacology, toxicology and functional effects, the preparation of optically pure chiral modular compounds is very important in medicine, pesticides, etc. , environmental protection, materials and other fields are of great significance. [0003] Optically pure chiral aryl o-diols, such as (S)-phenylethylene glycol, are not only indispensable chiral additives in liquid crystal materials, but also important intermediates for the synthesis of many optically active drugs and some functional materials. There are generally two synthetic methods for chiral aryl-o-diols. [0004] A kinetic resolution synthesis method for racemic diol. A disadvantage of this method is that its theoretical yield is only 50%. ...

AI Technical Summary

Problems solved by technology

However, there is a potential risk of plasmid loss during the subculture process of genetically engineered bacteria, resulting in poor subculture stability. During the cultivation process, expensive inducers such as isopropyl-β-D-thiogalactoside and antibiotics need to be added to cause fermentation. The cost of environmental treatment of waste liquid has increased, and it has not been applied to the synthesis of chiral aryl glycols with other structures

[0008] In summary, the preparation methods of chiral aryl vicinal diols are difficult to realize industrialization due to problems such as complex synthesis steps and unfriendly environment, or low optical purity and yield, or poor strain stability and narrow substrate spectrum.

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