A kind of rapid detection method of volatile substance content in traditional Chinese medicine steam distillate

A technology for steam distillation and volatile substances, which is applied in measuring devices, specific gravity measurement, instruments, etc., can solve the problems of expensive instruments, inaccurate quantification, and long inspection time, and achieves easy parallel operation, simple and fast operation, and test. Small sample size effect

Active Publication Date: 2017-02-22
CHIATAI QINGCHUNBAO PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method is simple and practical, there are also problems such as large sampling volume, long distillation time, inaccurate quantification, and susceptibility to environmental interference; another gas chromatography method has accurate measurement results, but the inspection time is too long and the instrument is expensive, so it is not suitable for Requirements for rapid inspection on the production site

Method used

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  • A kind of rapid detection method of volatile substance content in traditional Chinese medicine steam distillate
  • A kind of rapid detection method of volatile substance content in traditional Chinese medicine steam distillate
  • A kind of rapid detection method of volatile substance content in traditional Chinese medicine steam distillate

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Effect test

Embodiment 1

[0037]Precisely measure the dehydrated and refined volatile oil of Schisandra chinensis into a stoppered scale test tube, add pure water to make up to 40ml, prepare a series of suspensions with different concentrations as standard samples, and fully shake them on a vortex shaker to mix them. uniform. Schisandra volatile oil is operated according to the volatile substance determination method (distillation method) contained in an appendix XD of the 2010 version of "Chinese Pharmacopoeia", and the specific steps are shown in Example 2. The volatile oil obtained in the volatile oil tester is taken and dried over anhydrous sodium sulfate Dehydration, to obtain Schisandra volatile oil.

[0038] The volume concentration (V / V) of the volatile oil of Schisandra chinensis in this series of standard samples is 0%, 0.025%, 0.05%, 0.075%, 0.1%, 0.2%, respectively, and each concentration is parallel in triplicate. Add 4ml of saturated saline and 3ml of n-hexane to each standard sample, mi...

Embodiment 2

[0044] The contrast of embodiment 2 different assay methods

[0045] Get 3 parts of the Fructus Schisandra steam distillation sample in actual production, measure volatile oil content with rapid detection method of the present invention and 2010 editions " Chinese Pharmacopoeia " one appendix XD carried volatile matter determination method (distillation method) respectively.

[0046] The device of the method contained in the Pharmacopoeia is as follows:

[0047] A 1000ml hard round bottom flask is connected with a volatile oil tester, and the upper end of the volatile oil tester is connected with a reflux condenser. All the above parts are connected with glass grinding mouth. The volatile oil tester should have a scale of 0.1ml. All instruments should be fully cleaned, and check whether the joints are tight to prevent volatile oil from escaping.

[0048] The operation steps are: take 500mL of the test sample (approximately equivalent to 0.5-1.0ml of volatile oil), measure t...

Embodiment 3

[0052] Embodiment 3 Application example of the inventive method——Schisandra chinensis distillation process research

[0053] The method of the invention is intended to be applied to on-site monitoring and technological process research of steam distillation operations of traditional Chinese medicines. In this experimental example, the distillation process of Schisandra chinensis was taken as the research object to verify the time-effective advantage of the method of the present invention in field monitoring and process research.

[0054] Schisandra is a commonly used nourishing and strong traditional Chinese medicine, and its volatile substances are one of the main active ingredients. This experiment mainly takes Schisandra chinensis as the research object. Investigate the impact of direct steam distillation on extraction rate and production efficiency, the specific process is as follows:

[0055] Put 15kg of schisandra medicinal materials into the extraction tank, close the...

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Abstract

The invention discloses a method for quickly detecting content of volatile matter in a traditional Chinese medicinal steam distillate. The method includes the following steps: an extraction agent is used to extract the volatile matter from the traditional Chinese medicinal steam distillate, wherein the volatile matter is dissolved in the extraction agent, and therefore the density of an extraction liquid changes accordingly; a volatile matter standard sample is used to accurately measure the density of the extraction liquid, and then a standard curve is drawn; then the density of the extraction liquid is measured after the sample to be measured is extracted, and the content of the volatile matter in a distillate sample to be measured can be calculated in combination with the standard curve. The method provided by the invention is simple and fast in operation and short in measuring time; the method uses only a small sample amount, is easy to implement concurrent operations and high in accuracy, and is hardly affected by environmental conditions; the repeatability error is lower than 5%, and the reproducibility error is lower than 8%; a sample with a volatile matter content of at least 0.02% can be detected, and the accuracy and sensitivity are both higher than those of a distillation method.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine quality control and inspection and analysis, and is suitable for technological process research and on-site detection of traditional Chinese medicine steam distillation operation. Background technique [0002] Volatile substances are widely distributed in the plant kingdom. Hundreds of plants used as traditional Chinese medicine can extract volatile oils, especially in the families and genera such as Compositae, Rutaceae, Umbelliferae, and Zingiberaceae. The vast majority of volatile substances are insoluble in water but easily soluble in organic solvents. The chemical structure mainly includes aromatic compounds, terpenes, sesquiterpenes and their oxygen-containing derivatives, aliphatic compounds and so on. A large number of pharmacological experiments have proved that the volatile substances in medicinal plants have a wide range of effects, including anti-inflammatory, anti-allergic,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N9/36
Inventor 谭理妮刘雳胡建伟胡济宏孔万明
Owner CHIATAI QINGCHUNBAO PHARMA
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