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A kind of high performance liquid chromatography analysis method of serine

A high-performance liquid chromatography and analysis method technology, applied in the field of component analysis, to achieve the effects of accurate quantitative analysis, accurate detection results, and reliable methods

Active Publication Date: 2016-04-20
南京仁为医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Since serine has no absorption in the ultraviolet region, it needs to be equipped with an evaporative light scattering detector or a mass spectrometer for detection by liquid chromatography, and cannot be directly detected by a commonly used ultraviolet detector

Method used

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  • A kind of high performance liquid chromatography analysis method of serine
  • A kind of high performance liquid chromatography analysis method of serine
  • A kind of high performance liquid chromatography analysis method of serine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Detect the content of serine in the sample

[0038] Calculation of sample concentration: by comparing the serine response value of the sample pyridine derivative solution in the chromatogram with the response value of the standard pyridine derivative solution, the content of serine in the tested sample is converted. The peak areas of the serine pyridine derivatives of the standard and sample in the chromatogram were averaged to obtain the average value of the peak areas. Calculate the mass fraction of serine in the sample according to formula 1:

[0039] w % = m 1 × A 2 × P m 2 × A 1 × 100 %

[0040] Formula 1 ...

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Abstract

The invention discloses a high performance liquid chromatographic analysis method of serine, belonging to the technical field of analysis. According to the method, derivatization is carried out on the serine by feeding 2-chloro pyridine, a serine standard solution and a serine sample solution are respectively prepared after derivatization, the sample solution and the standard solution are respectively detected by a high-performance liquid chromatography, and the standard substance, the sample and the standard substance are sequentially detected. After the method is adopted, rapid and accurate quantitative analysis is carried out on the serine sample, so that a reaction process is monitored. The method has the characteristics that a derivatization reagent can be obtained easily, detection results are accurate and reliability is realized.

Description

technical field [0001] The invention relates to the technical field of component analysis, in particular to a high-performance liquid chromatography analysis method for serine. Background technique [0002] Serine is a non-essential amino acid, its chemical name is: 2-amino-3-hydroxypropionic acid, and its molecular formula is C 3 h 7 NO 3 , the molecular weight is 105.09, and the structural formula is as follows: [0003] [0004] Since serine has no absorption in the ultraviolet region, detection by liquid chromatography needs to be equipped with an evaporative light scattering detector or a mass spectrometer detector, and it cannot be directly detected by a commonly used ultraviolet detector. The detection methods reported in related literature at home and abroad include: serine and ninhydrin chromogenic spectrophotometry, capillary electrophoresis, dinitrobenzene derivatization method, and fluorescence detection method. Contents of the invention [0005] The pur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 肖云刘力萍徐强薛谊李维思韩小军
Owner 南京仁为医药科技有限公司