Asynchronous high-speed countercurrent chromatography instrument with multiple separation columns

Abstract

The invention discloses an asynchronous high-speed countercurrent chromatography instrument with multiple separation columns. The asynchronous high-speed countercurrent chromatography instrument comprises a rotation bracket installed in a chassis, wherein the rotation bracket is provided with at least one separation column, and one side of the chassis is provided with a complete-revolution motor for completely revolving the separation column and an autorotation motor for automatically rotating the separation column; the complete-revolution motor and the autorotation motor are independently controlled, and various different centrifugal force fields are provided by individually adjusting the autorotation and complete-revolution speeds of the separation column and the rotation direction. Different isolating environment is provided to separated samples by adjusting the autorotation and complete-revolution speeds, in comparison with a traditional high-speed countercurrent chromatography instrument, the asynchronous high-speed countercurrent chromatography instrument is unwound by a rotary sealing joint instead of a flexible hose, thus the asynchronous high-speed countercurrent chromatography instrument is characterized in that multiple gears cannot be used for unwinding, the structure is simplified, an intermediate shaft is eliminated, the beta value of the separation column is ensured, and one or more separation columns can be selectively installed.

Description

technical field [0001] The invention relates to a high-speed countercurrent chromatograph using the principle of liquid-liquid distribution and separation applied in the field of biochemistry, and in particular discloses a high-speed countercurrent chromatograph with asynchronous planetary rotation and multi-separation columns. Background technique [0002] High-speed countercurrent chromatography is a separation instrument that uses liquid-liquid extraction as the separation theory and is used in the fields of biology, chemistry, and traditional Chinese medicine. The phase solvents are mixed continuously, the sample is distributed repeatedly, and the samples are eluted sequentially according to the distribution coefficient. Because there is no need for any solid carrier or support, it can achieve high-efficiency separation and preparation in a short time, and can reach thousands of theoretical plates. Compared with other types of separation chromatography, it not only over...

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Problems solved by technology

[0003] At present, various existing high-speed countercurrent chromatography is positioned as a general-purpose high-speed countercurrent chromatography, that is to say, different types of samples are used on the same high-speed countercurrent chromatography. Due to the difference in interfacial tension of different types of solvent systems, specific gravity and viscosity The difference makes some types of samples have better separation effect, and some types of samples have poor separation effect, which greatly reduces the use effect and efficiency of high-speed countercurrent chromatography, and cannot give full play to the advantages of high-speed countercurrent chromatography in

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