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Water-soluble biological nematicide fermentation method and application

A biological nematicide and a fermentation method technology are applied in the field of fermentation of water-soluble biological nematicidal pesticides to achieve the effects of low energy consumption, strong activity and no toxic and side effects

Inactive Publication Date: 2015-05-06
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the increasing severity of plant parasitic nematode diseases and the enhancement of people's environmental and health awareness, there is a great demand for biopesticides for the control of plant nematode diseases at home and abroad, but there is a large gap in suitable biocontrol agents in this regard

Method used

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  • Water-soluble biological nematicide fermentation method and application
  • Water-soluble biological nematicide fermentation method and application
  • Water-soluble biological nematicide fermentation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0148] Fermentation method of water-soluble biological nematicide pesticide concentrated water:

[0149] (1) Activation culture of Sr18 bacteria and preparation of spore suspension: put the strains preserved in the ampoule tube into the slant medium, and culture at 26°C for 6 days; take the activated Sr18 slant, add 5ml of sterile water to wash the lower slant Pour the spores on the glass beads into the Erlenmeyer flask, shake for 1 minute, and count;

[0150] (2) 200L tank fermentation enlargement: use 50L fermentation tank as seed tank to prepare first-grade seeds: according to 2.75×10 6 / L spore concentration Put the spore suspension prepared in step (1) into a seed tank filled with 22L medium, and incubate at 26°C for 24h. Put the first-grade seeds into a 200L fermenter with 150L fermentation medium at a 4% inoculum volume, stirring at a speed of 198r / min, tank pressure at 0.05MPa, ventilation to maintain a dissolved oxygen level of 20%, and a fermentation time of 40 hour...

Embodiment 2

[0154] The fermentation method of water-soluble biological nematicide powder is characterized in that it is carried out according to the following steps:

[0155] (1) Activation culture of Sr18 bacteria and preparation of spore suspension: put the strains preserved in the ampoule tube into the slant medium, and culture at 26°C for 6 days; take the activated Sr18 slant, add 5ml of sterile water to wash the lower slant Pour the spores on the glass beads into the Erlenmeyer flask, shake for 1 minute, and count;

[0156] (2) 200L tank fermentation enlargement: use 50L fermentation tank as seed tank to prepare first-grade seeds: according to 2.75×10 6 / L spore concentration Put the spore suspension prepared in step (1) into a seed tank filled with 22L medium, and incubate at 26°C for 24h. Put the first-grade seeds into a 200L fermenter with 150L fermentation medium at a 4% inoculum volume, stirring at a speed of 198r / min, tank pressure at 0.05MPa, ventilation to maintain a dissolv...

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Abstract

The invention discloses a water-soluble biological nematicide fermentation method and application. The fermentation method comprises the steps of Sr18 bacterium activating culture, 200L tank fermentation scale up, 5t tank fermentation scale up, concentrated water preparation, powder preparation and the like. The experimental results show that the fermentation process established by the invention is secondary fermentation, and has the characteristics of simple and easy fermentation process control, short fermentation period, low energy consumption, high yield of active products and the like; the prepared concentrated water has strong activity, no toxic side effects, stability and convenient storage, and the shelf life is more than two years; the preparation is convenient to apply, can be mixed with water according to any proportion, and can also be matched with other pesticides. The biological nematicide obtained by fermentation has a specificity effect on prevention of Meloidogyne incognita Chitwood, Bursaphelenchus xylophilus and vegetable root-knot nematode diseases.

Description

[0001] This invention was funded by the key project of Tianjin Science and Technology Research Program (Project No. 05YFGZGX04400). technical field [0002] The invention belongs to the technical field of biological fermentation, and relates to a fermentation method and application of water-soluble biological nematode pesticides. Background technique [0003] Plant nematodes are a kind of important plant pathogens distributed worldwide. They can parasitize the roots, stems, leaves, flowers, buds and seeds of plants. They have strong environmental adaptability. Almost every plant can be infected by one or several nematodes. Parasitism or invasion causes various nematode diseases in plants, which brings serious harm to the development of agriculture and forestry. The damaged plants' own resistance is reduced, and other pathogens take the opportunity to invade plants and form complex diseases with nematodes, resulting in reduced yields, withered plants, and even death, causing ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
CPCA01N63/30C12N1/14
Inventor 孙建华孟庆恒冯欣任毅田中原宇克莉杜连祥
Owner TIANJIN NORMAL UNIVERSITY
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