Royal jelly protein polypeptide
A technology of pro milk protein and protease hydrolyzate, applied in the field of pro milk protein polypeptide, can solve the problems of difficult absorption of nutrients and difficulty in fully exerting potential effects, and achieves the effect of promoting skin elasticity and excellent free radical scavenging effect.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0027] Example 1
[0028] Preparation of honey protein peptides:
[0029] 1) Take 100g royal jelly, add 800g saturated sodium chloride solution, let stand for 1.5h, centrifuge at 5000rpm for 5min, take the supernatant; adjust the pH of the supernatant to 4.0 with hydrochloric acid, stand for 1.5h at 8000rpm Centrifuge for 10 min, discard the supernatant; wash the precipitate 4 times with pH 4.0 hydrochloric acid, after each wash, centrifuge at 8000 rpm for 10 min, discard the supernatant, and get propolis protein;
[0030] 2) Add 15 times the volume of a 0.35% dilute hydrochloric acid solution to the honey protein obtained in step 1) to obtain the honey protein solution;
[0031] 3) Put the propolis protein solution of step 2) in a 37°C water bath, add pepsin with a specific activity of 3000 U / g to make the final concentration of pepsin 0.3g / ml, and fully enzymolyze to obtain pepsinase Solution
[0032] 4) Adjust the pH of the pepsin hydrolysate obtained in step 3) to 7.0, adjust the ...
Example Embodiment
[0034] Example 2
[0035] Preparation of honey protein peptides:
[0036] 1) Take 100g royal jelly, add 500g saturated sodium chloride solution, let stand for 1h, centrifuge at 4800rpm for 3min, take the supernatant; adjust the pH of the supernatant to 3.8 with hydrochloric acid, let stand for 1h, centrifuge at 7800rpm for 8min , Discard the supernatant; wash the precipitate 3 times with pH 3.8 hydrochloric acid, after each wash, centrifuge at 7800 rpm for 8 min, discard the supernatant, and get propolis protein;
[0037] 2) Add 10 times the volume of a 0.30% dilute hydrochloric acid solution to the propolis protein obtained in step 1) to obtain a propolis protein solution;
[0038] 3) Put the honey protein solution of step 2) in a 36°C water bath, add pepsin with a specific activity of 2500 U / g to make the final concentration of pepsin 0.2g / ml, and fully enzymolyse to obtain pepsin enzyme Solution
[0039] 4) Adjust the pH of the pepsin hydrolysate obtained in step 3) to 6.0, adjust ...
Example Embodiment
[0041] Example 3
[0042] Preparation of honey protein peptides:
[0043] 1) Take 100g royal jelly, add 1000g saturated sodium chloride solution, let stand for 2h, centrifuge at 5200rpm for 8min, take the supernatant; adjust the pH of the supernatant to 4.2 with hydrochloric acid, let stand for 2h, centrifuge at 8200rpm for 12min , Discard the supernatant; wash the precipitate 5 times with pH 4.2 hydrochloric acid, after each wash, centrifuge at 8200 rpm for 12 min, discard the supernatant, and get propolis protein;
[0044] 2) Add 20 times the volume of a 0.4% dilute hydrochloric acid solution to the honey protein obtained in step 1) to obtain the honey protein solution;
[0045] 3) Put the propolis protein solution of step 2) in a 38℃ water bath, add pepsin with a specific activity of 4000U / g to make the final concentration of pepsin 0.4g / ml, and fully enzymolyse to obtain pepsinase Solution
[0046] 4) Adjust the pH of the pepsin hydrolysate obtained in step 3) to 8.0, adjust the t...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap