Collagen transplanting material with cartilage and preparation method for collagen transplanting material

Abstract

The invention discloses a collagen transplanting material with cartilage and a preparation method for the collagen transplanting material, and belongs to the biomedical field. The preparation method for the collagen transplanting material with cartilage comprises the following steps: (1) getting bone joints, and sealing articular cartilage of the bone joints with a tissue embedding medium; (2) putting the bone joints processed in the step (1) into 8-12% (mass percent concentration) formic acid and soaking for 3-4 days at 4-8 DEG C, and flushing the soaked bone joints; (3) removing the tissue embedding medium and cleaning the articular cartilage to obtain the collagen transplanting material with cartilage. The collagen transplanting material with cartilage prepared by the preparation method disclosed by the invention comprises a bracket consisting of bone collagens, is provided with active cartilage tissue, can promote regenerative cartilage formation and is beneficial to bone tissue generation.

Description

technical field [0001] The invention relates to a graft material and a preparation method thereof, in particular to a collagen graft material with cartilage and a preparation method thereof. Background technique [0002] In bone tumors, artificial joint replacement or plastic surgery, there is no suitable graft material, so there is no ideal treatment plan for this kind of disease clinically. A suitable graft material needs to meet two requirements at the same time: bone scaffold and active cartilage tissue. Autologous bone transplantation is difficult to be widely used due to limited sources and destructiveness; allogeneic bone is an antigen at the same time, causing immune reactions in the body after transplantation. Inhibitors, etc., can alleviate the rejection reaction to a certain extent, but as time goes by, the transplanted joints will degenerate, and even the joint structure will be completely destroyed and lose its function. [0003] It is extremely difficult to r...

AI Technical Summary

Problems solved by technology

Autologous bone transplantation is difficult to be widely used due to limited sources and destructiveness; allogeneic bone is an antigen at the same time, causing immune reactions in the body after transplantation. Inhibitors, etc., can alleviate the rejection reaction to a certain extent, but as time goes by, the transplanted joints will degenerate, and even the joint structure will be completely destroyed and lose its function.

[0003] It is extremely difficult to repair cartilage tissue after injury. Tissue engineered cartilage transplantation is a new technology for the treatment of cartilage defects. However, there are still problems in the integration of repaired tissue and normal cartilage boundaries, the formation of new cartilage tissue composed of chondrocytes and scaffold materials, and Functionality, and the ability to withstand mechanical loads, etc.

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