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A kind of collagen transplant material with cartilage and its preparation method

A technique of grafting materials and collagen, applied in medical science, prosthesis, etc., can solve problems such as loss of function, limited source, and damage to joint structure, and achieve the effect of facilitating the generation and promoting the formation of new cartilage

Active Publication Date: 2017-01-25
广州市红十字会医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Autologous bone transplantation is difficult to be widely used due to limited sources and destructiveness; allogeneic bone is an antigen at the same time, causing immune reactions in the body after transplantation. Inhibitors, etc., can alleviate the rejection reaction to a certain extent, but as time goes by, the transplanted joints will degenerate, and even the joint structure will be completely destroyed and lose its function.
[0003] It is extremely difficult to repair cartilage tissue after injury. Tissue engineered cartilage transplantation is a new technology for the treatment of cartilage defects. However, there are still problems in the integration of repaired tissue and normal cartilage boundaries, the formation of new cartilage tissue composed of chondrocytes and scaffold materials, and Functionality, and the ability to withstand mechanical loads, etc.

Method used

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  • A kind of collagen transplant material with cartilage and its preparation method
  • A kind of collagen transplant material with cartilage and its preparation method
  • A kind of collagen transplant material with cartilage and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0035] An embodiment of the collagen graft material with cartilage and its preparation method of the present invention. The collagen graft material with cartilage in this embodiment is prepared by the following method:

[0036] (1) Take a 1.8-2.2 cm long adult rabbit proximal radius joint and wash it with a phosphate buffer solution with a pH of 7.4; then, heat the tissue embedding medium to melt (heating temperature is about 56°C) , The articular cartilage of the proximal radius joint of adult rabbits is quickly immersed in the tissue embedding medium and then taken out immediately, and the tissue embedding medium on the surface of the articular cartilage is naturally cooled to seal the articular cartilage;

[0037] (2) Place the bone joints treated in step (1) in formic acid with a concentration of 8-12% by mass, and soak at 4-8℃ for 3 to 4 days until the bone is completely softened. Use phosphoric acid with a pH of 7.4 Rinse the soaked bone and joint with salt buffer solution;

...

Embodiment 2

[0040] An embodiment of the collagen graft material with cartilage and its preparation method of the present invention. The collagen graft material with cartilage in this embodiment is prepared by the following method:

[0041] (1) Take a 1.8-2.2 cm long adult rabbit’s proximal radius joint and wash it with normal saline; then, heat the tissue embedding medium to melt (heating temperature is about 56°C), and remove the adult rabbit’s proximal radius The articular cartilage of the bone joint is quickly immersed in the tissue embedding medium and then taken out immediately, and the tissue embedding medium on the surface of the articular cartilage is naturally cooled to seal the articular cartilage;

[0042] (2) Place the bone joints treated in step (1) in formic acid with a concentration of 8-12% by mass, soak them at 4-8°C for 3 to 4 days until the bones are completely softened, and rinse the soaked bones with normal saline. bone joint;

[0043] (3) Gently peel off the tissue embeddi...

Embodiment 3

[0045] In order to investigate the structure of the collagen graft material with cartilage of the present invention, we sprayed platinum on the surface of the collagen graft material with cartilage, and then placed it under a scanning electron microscope to observe the surface structure of the cartilage tissue and collagen scaffold. The structural appearance of the collagen graft material with cartilage of the present invention is as Figure 1a Shown ( Figure 1a The box in the box shows the part of the articular cartilage that needs to be sealed and protected), and the ultrastructure of the cartilage tissue is shown in Figure 1b , See the ultrastructure of the collagen scaffold Figure 1c .

[0046] The results show that the collagen scaffold of the collagen graft material with cartilage of the present invention has uneven natural voids (see Figure 1c ).

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Abstract

The invention discloses a collagen transplanting material with cartilage and a preparation method for the collagen transplanting material, and belongs to the biomedical field. The preparation method for the collagen transplanting material with cartilage comprises the following steps: (1) getting bone joints, and sealing articular cartilage of the bone joints with a tissue embedding medium; (2) putting the bone joints processed in the step (1) into 8-12% (mass percent concentration) formic acid and soaking for 3-4 days at 4-8 DEG C, and flushing the soaked bone joints; (3) removing the tissue embedding medium and cleaning the articular cartilage to obtain the collagen transplanting material with cartilage. The collagen transplanting material with cartilage prepared by the preparation method disclosed by the invention comprises a bracket consisting of bone collagens, is provided with active cartilage tissue, can promote regenerative cartilage formation and is beneficial to bone tissue generation.

Description

Technical field [0001] The invention relates to a graft material and a preparation method thereof, in particular to a collagen graft material with cartilage and a preparation method thereof. Background technique [0002] In bone tumors, artificial joint replacement or plastic surgery, there is no suitable transplant material, so there is no ideal treatment plan for this kind of disease in clinic. Appropriate graft materials need to meet two requirements at the same time: bone scaffold and active cartilage tissue. Autologous bone transplantation is difficult to be widely used due to its limited source and destructive nature; allogeneic bone is also an antigen, which causes an immune response in the body after transplantation. Although it is processed by various methods, such as radiation, freeze-drying, deep hypothermia, and postoperative application Inhibitors, etc., can alleviate rejection to a certain extent, but as time goes by, the grafted joints all undergo degeneration, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50
Inventor 李斯明杨小红崔树良
Owner 广州市红十字会医院
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