Preparation method of chitosan attapulgite membrane material having function of guiding bone tissue regeneration

A technology of bone tissue regeneration and attapulgite, applied in tissue regeneration, prosthesis, medical science, etc., can solve the problems of unfavorable cell migration and tissue formation, small pores of electrospun nanomaterials, and easy to produce immune rejection, etc. To achieve the effect of promoting the elimination of metabolites, good degradability, and promoting supply

Active Publication Date: 2021-06-08
CHANGZHOU NO 2 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the repairing effect of autologous bone grafting is obviously better than that of allograft bone grafting, autologous bone grafting has limited sources of materials and cannot meet clinical needs; although allograft bone sources are abundant, there are many shortcomings: ① prone to immune rejection; ② may carry heterogeneous bone source pathogens, there is a risk of zoonosis; ③There are certain ethical issues
[0005] There are many methods for the preparation of membrane materials, among which high-voltage electrospinning technology is one of the most commonly used methods. Although this technology has the advantages of relatively cheap equipment, simple operation and many electrospun materials, electrospun nanomaterials have small pores. Disadvantages cell migration and tissue formation

Method used

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  • Preparation method of chitosan attapulgite membrane material having function of guiding bone tissue regeneration
  • Preparation method of chitosan attapulgite membrane material having function of guiding bone tissue regeneration
  • Preparation method of chitosan attapulgite membrane material having function of guiding bone tissue regeneration

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Effect test

Embodiment 1

[0029] A preparation method of a chitosan attapulgite membrane material with guided bone tissue regeneration, comprising the following steps:

[0030] Step 1: According to the mass ratio of 90:10, take chitosan and attapulgite respectively, dissolve chitosan in 0.2% acetic acid solution by mass percentage, mix well, add attapulgite, and stir the mixture at a speed of 250r / min 24h, the mixture was obtained;

[0031] Step 2: Slowly add the mixed solution obtained in step 1 to a volume of 50cm 3 Pre-cool in a plastic mold at 4°C for 12 hours, then freeze at -20°C for 24 hours;

[0032] Step 3: Slowly add a mixture of 2M NaOH and 100% ethanol at a mass molar ratio of 1:1, and freeze at -20°C for 24 hours to obtain a membrane material;

[0033] Step 4: wash the membrane material obtained in step 3 with 0.2M PBS (pH=7.4) in molar mass for 3 to 5 times / 30min, and then use ethanol solutions with mass percentages of 50, 70, 80, 90, 95 and 100% respectively in sequence Gradient dehyd...

Embodiment 2

[0036] A preparation method of a chitosan attapulgite membrane material with guided bone tissue regeneration, comprising the following steps:

[0037] Step 1: According to the mass ratio of 80:20, take chitosan and attapulgite respectively, dissolve chitosan in 0.2% acetic acid solution by mass percentage, mix well, add attapulgite, and stir the mixture at a speed of 250r / min 24h, the mixture was obtained;

[0038] Step 2: Slowly add the mixed solution obtained in step 1 to a volume of 50cm 3 Pre-cool in a plastic mold at 4°C for 12 hours, then freeze at -20°C for 24 hours;

[0039] Step 3: Slowly add a mixture of 2M NaOH and 100% ethanol at a mass molar ratio of 1:1, and freeze at -20°C for 24 hours to obtain a membrane material;

[0040] Step 4: The membrane material obtained in step: 3 is washed 3 to 5 times / 30min with the mass molar number 0.2M PBS (pH=7.4), and then successively with 50, 70, 80, 90, 95 and 100% ethanol solutions by mass percentage Gradient dehydration,...

Embodiment 3

[0043] A preparation method of a chitosan attapulgite membrane material with guided bone tissue regeneration, comprising the following steps:

[0044] Step 1: According to the mass ratio of 70:30, take chitosan and attapulgite respectively, dissolve chitosan in 0.2% acetic acid solution by mass percentage, mix well, add attapulgite, and stir the mixture at a speed of 250r / min 24h, the mixture was obtained;

[0045] Step 2: Slowly add the mixed solution obtained in step 1 to a volume of 50cm 3 Pre-cool in a plastic mold at 4°C for 12 hours, then freeze at -20°C for 24 hours;

[0046]Step 3: Slowly add a mixture of 2M NaOH and 100% ethanol at a mass molar ratio of 1:1, and freeze at -20°C for 24 hours to obtain a membrane material;

[0047] Step 4: Wash the membrane material obtained in step 3 with 0.2M PBS (pH=7.4) in mass molarity for 3 to 5 times / 30min, and then use 50, 70, 80, 90, 95 and 100% ethanol solutions with mass percentages to gradually gradient Dehydration, and f...

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Abstract

The invention provides a preparation method of a chitosan attapulgite membrane material having function of guiding bone tissue regeneration. The preparation method comprises the following preparation steps: dissolving chitosan in an acetic acid solution to obtain a chitosan acetic acid solution; adding attapulgite, and performing uniform mixing to obtain a chitosan/attapulgite mixed solution; putting the chitosan/attapulgite mixed solution in a container for film formation, then performing Gradient temperature cooling until freezing, and adding an ethanol/sodium hydroxide mixed solution for further freezing for several hours. and performing washing with a buffer solution, carrying out gradient alcohol dehydration, washing the membrane with a glycerol/aqueous solution, and performing natural drying at room temperature to obtain the chitosan attapulgite membrane material having the function of guiding bone tissue regeneration. The nanofiber membrane material prepared by the invention has good flexibility, degradability, biocompatibility and bone regeneration guiding function, has no toxic or side effect, and meets the requirements of tissue engineering materials.

Description

technical field [0001] The invention relates to the field of bone tissue regeneration and repair biomaterials, in particular to a preparation method of a chitosan attapulgite membrane material capable of guiding bone tissue regeneration. Background technique [0002] There are more than 3 million patients with bone defects or dysfunction in my country every year. Bone injury or bone defect is very common clinically. At present, autologous bone or allogeneic bone transplantation is mainly used to promote bone regeneration and repair. Although the repairing effect of autologous bone grafting is obviously better than that of allograft bone grafting, autologous bone grafting has limited sources of materials and cannot meet clinical needs; although allograft bone sources are abundant, there are many shortcomings: ① prone to immune rejection; ② may carry heterogeneous bone source pathogens, there is a risk of zoonosis; ③ there are certain ethical issues. In order to overcome the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/02A61L27/20A61L27/50A61L27/56A61L27/58
CPCA61L27/20A61L27/025A61L27/50A61L27/56A61L27/58A61L2430/02C08L5/08
Inventor 赵红斌周栋马佳义刘均吴思宇戴婷张震宇
Owner CHANGZHOU NO 2 PEOPLES HOSPITAL
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