Tree shrew retina ischemic injury experiment model adopting photochemical induction

Abstract

The invention relates to a tree shrew retina ischemic injury experiment model adopting photochemical induction, and belongs to the technical field of clinical medicine, pharmacy and cell biology. The experiment model is obtained through steps as follows: a healthy adult tree shrew is selected and is anaesthetized by 40 mg*kg(-1) of 2.5% sodium thiopental through intraperitoneal injection; the tree shrew is fixed on an operating table, and 2.75% rose Bengal normal saline is injected from the sublingual vein of the animal in a dose of 0.031 ml / 10 g; 2 drips or 3 drips of a 0.25% compound tropicamide solution are utilized to dilate pupils; after rose Bengal is circulated for 10 min, a small animal cerebral thrombosis experimental device with the patent number of ZL201420068737.2 is adopted to irradiate a left eyeball or a right eyeball, so that vascular endothelial injury and platelet aggregation induce thrombogenesis, and the surface temperature of the irradiated part is controlled to be 37.0 DEG C plus or minus 0.1 DEG C; the thermal insulation is performed, the animal is placed back to a cage after coming round, fundus retina photographing is performed after the experiment is finished for 4 h, 24 h and 72 h; fundus artery thrombogenesis and retina injury change are dynamically observed with a conventional method. The model has the advantages of simplicity and convenience in operation, good repeatability and low experiment cost.

Description

technical field [0001] The invention relates to an experimental model of photochemically induced tree shrew retinal ischemic damage, in particular to an experimental model for simulating human retinal ischemic damage, belonging to the technical fields of clinical medicine, pharmacy and cell biology. Background technique [0002] Retinal tissue metabolism is extremely vigorous, and the blood supply of its inner layer comes from retinal blood vessels with terminal branches, so it is very prone to ischemic lesions. Since the retinal tissue is where the optic neurons are located, once ischemia occurs, it will do great harm to the visual function, and it is also a common cause of blindness and visual impairment caused by diseases such as glaucoma, retinal artery occlusion, and diabetic retinopathy. Concerns with basic medicine. Therefore, replicating corresponding experimental animal models is of great value in revealing the mechanism of retinal ischemic injury and then effectiv...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the existing experimental model of retinal ischemia, such as poor animal selection and simulation, large mechanical damage, susceptibility to infection, and high animal mortality, and provide an experimental model of photochemically induced tree shrew retinal ischemic damage